β-1,3-Gal-T3 Inhibitors
Chemical inhibitors of β-1,3-Galactosyltransferase 3 can significantly disrupt the enzyme's function through various mechanisms that impact the glycosylation pathway. Tunicamycin inhibits the initial step of N-linked glycosylation, preventing the addition of N-acetylglucosamine to dolichol phosphate. This early blockade can prevent proper folding and trafficking of glycoproteins, which are necessary for β-1,3-Galactosyltransferase 3 to function effectively. Similarly, Swainsonine interferes with glycoprotein processing by inhibiting α-mannosidase II. This results in the accumulation of glycoproteins with improper glycan structures, subsequently affecting the activity of β-1,3-Galactosyltransferase 3 due to the lack of mature substrates.
Furthermore, Castanospermine and Deoxynojirimycin, both glucosidase inhibitors, impede the trimming of glucose residues necessary for the maturation of glycoproteins. The result is an accumulation of misfolded glycoproteins not recognized by β-1,3-Galactosyltransferase 3, leading to an indirect inhibition of the enzyme's activity. Deoxymannojirimycin operates in a similar manner by inhibiting mannosidase I, causing an improper trimming of mannose residues, which also results in misfolded glycoproteins that are not suitable substrates for β-1,3-Galactosyltransferase 3. Brefeldin A, Nocodazole, and Colchicine disrupt intracellular transport and Golgi apparatus function, which are essential for the localization and operation of β-1,3-Galactosyltransferase 3. Monensin alters ion gradients within the Golgi, affecting the enzyme's environment and thereby its function. Collectively, these inhibitors can lead to a significant reduction in the functional activity of β-1,3-Galactosyltransferase 3 by targeting different aspects of glycoprotein processing and trafficking within the cell.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $172.00 $305.00 | 66 | |
This chemical inhibits N-linked glycosylation by blocking the transfer of N-acetylglucosamine to dolichol phosphate. β-1,3-Gal-T3 is involved in the glycosylation process, and inhibition of N-linked glycosylation can disrupt its function by preventing proper substrate processing or by altering glycoprotein folding and trafficking, which are essential for β-1,3-Gal-T3 activity. | ||||||
Swainsonine | 72741-87-8 | sc-201362 sc-201362C sc-201362A sc-201362D sc-201362B | 1 mg 2 mg 5 mg 10 mg 25 mg | $138.00 $251.00 $631.00 $815.00 $1832.00 | 6 | |
Swainsonine inhibits mannosidase II, an enzyme involved in glycoprotein processing; this could lead to improper glycoprotein maturation and function. Since β-1,3-Gal-T3 is responsible for transferring galactose in the biosynthesis of complex glycoproteins, the inhibition of mannosidase II can interrupt the glycosylation pathway, indirectly inhibiting β-1,3-Gal-T3 function. | ||||||
Castanospermine | 79831-76-8 | sc-201358 sc-201358A | 100 mg 500 mg | $184.00 $632.00 | 10 | |
This chemical is an inhibitor of glucosidase I and II, enzymes that are necessary for the proper trimming of glycoproteins during maturation. By inhibiting these enzymes, castanospermine can prevent the correct formation of glycoprotein substrates that β-1,3-Gal-T3 acts upon, thereby inhibiting the function of β-1,3-Gal-T3. | ||||||
Deoxynojirimycin | 19130-96-2 | sc-201369 sc-201369A | 1 mg 5 mg | $73.00 $145.00 | ||
Deoxynojirimycin inhibits glucosidase I and II, similar to castanospermine. The inhibition of these enzymes hinders the correct folding and processing of glycoproteins, which is a prerequisite for the function of β-1,3-Gal-T3, thus indirectly inhibiting its activity. | ||||||
Deoxymannojirimycin hydrochloride | 84444-90-6 | sc-201360 sc-201360A | 1 mg 5 mg | $93.00 $239.00 | 2 | |
As an inhibitor of mannosidase I, this chemical disrupts the glycosylation process by preventing the removal of mannose residues, leading to misfolded glycoproteins. Since β-1,3-Gal-T3 requires correctly folded glycoprotein substrates for its enzymatic activity, the inhibition of mannosidase I by deoxymannojirimycin can result in functional inhibition of β-1,3-Gal-T3. | ||||||
Kifunensine | 109944-15-2 | sc-201364 sc-201364A sc-201364B sc-201364C | 1 mg 5 mg 10 mg 100 mg | $135.00 $540.00 $1025.00 $6248.00 | 25 | |
Kifunensine is a mannosidase I inhibitor that can disrupt the trimming of mannose residues in the glycosylation process. This inhibition can result in glycoprotein misfolding and can prevent β-1,3-Gal-T3 from properly functioning due to the lack of appropriately processed glycoprotein substrates. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $31.00 $53.00 $124.00 $374.00 | 25 | |
Brefeldin A disrupts the Golgi apparatus function and protein secretion by inhibiting the formation of the Golgi complex. Since β-1,3-Gal-T3 operates within the Golgi apparatus to transfer galactose moieties, disruption of Golgi function by Brefeldin A can lead to the functional inhibition of β-1,3-Gal-T3. | ||||||
Nocodazole | 31430-18-9 | sc-3518B sc-3518 sc-3518C sc-3518A | 5 mg 10 mg 25 mg 50 mg | $59.00 $85.00 $143.00 $247.00 | 38 | |
Nocodazole disrupts microtubule polymerization, which can affect the intracellular transport and proper localization of proteins to the Golgi apparatus. β-1,3-Gal-T3, being a Golgi-resident enzyme, relies on proper trafficking to the Golgi; thus, nocodazole can inhibit its function by impairing protein transport to the Golgi. | ||||||
Monensin A | 17090-79-8 | sc-362032 sc-362032A | 5 mg 25 mg | $155.00 $525.00 | ||
Monensin is an ionophore that disrupts Golgi function by altering ion gradients within the Golgi apparatus. The alteration in the environment within the Golgi can affect the activity of enzymes like β-1,3-Gal-T3 that are dependent on the Golgi's microenvironment for their proper function. | ||||||
Colchicine | 64-86-8 | sc-203005 sc-203005A sc-203005B sc-203005C sc-203005D sc-203005E | 1 g 5 g 50 g 100 g 500 g 1 kg | $100.00 $321.00 $2289.00 $4484.00 $18207.00 $34749.00 | 3 | |
Colchicine binds to tubulin and prevents microtubule assembly, which can inhibit intracellular trafficking. This can lead to a failure in the delivery of necessary proteins and substrates to the Golgi apparatus, where β-1,3-Gal-T3 resides, thus functionally inhibiting the enzyme. | ||||||