Kifunensine CAS: 109944-15-2
MF: C8H12N2O6
MW: 232.19

Kifunensine (CAS 109944-15-2)

Kifunensine | CAS 109944-15-2 is rated 5.0 out of 5 by 2.
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Alternate Names: Kifunensine is also known as KIF; FR 900494; hexahydro-6R,7S,8aS-trihydroxy-5R-(hydroxymethyl)-imidazo[1,2-a]pyridine-2,3-dione
Application: Kifunensine is an immunoactive cell adhesion inhibitor that displays competitive inhibition against immunosuppressive factor in mice
CAS Number: 109944-15-2
Purity: ≥98%
Molecular Weight: 232.19
Molecular Formula: C8H12N2O6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Kifunensine is an immunoactive compound originally produced by Kitasatosporia kifunense. Kifunensine displays competitive inhibition against immunosuppressive factor in tumor-bearing mice. Kifunensine has also been shown to be a potent inhibitor of the purified glycoprotein processing enzyme, mannosidase I (MAN1), specifically MAN1A1, MAN1A2, MAN1B1 and MAN1C1. Enzymes of this class are important factors for the biosynthesis of various types of N-linked oligosaccharide structures. Inhibition of these structures by kifunensine can interfere with cell-to-cell adhesion, targeting of lysosomal hydrolases to lysosomes, and clearance of asialoglycoproteins from the serum. Kifunensine inhibits human endoplasmic reticulum α-1,2-mannosidase I as well as members of the Golgi subfamily of the class I mannosidases (Golgi α-mannosidase IA, IB, and IC) exhibiting Ki values of 130 and 23 nM, respectively.


References

1. Iwami, M., et al. 1987. J. Antibiot. 40: 612-622. PMID: 3610820

2. Elbein, A.D., et al. 1990. J. Biol. Chem. 265: 15599-15605. PMID: 2144287

3. Elbein, A.D., et al. 1991. Arch. Biochem. Biophys. 288: 177-184. PMID: 1898016

4. Elbein, A.D. 1991. FASEB J. 5: 3055-3063. PMID: 1743438

Usage :
Stable for up to 1 year after receipt when stored at -20°C. Stock solutions should be aliquoted and stored at -20°C for up to 1 month.
Physical State :
Solid
Solubility :
Soluble in water (double-distilled, 50 mM), ethanol, DMF and DMSO.
Storage :
Store at -20° C
Refractive Index :
n20D 1.71
IC50 :
mung bean α-1,2-mannosidase I : IC50 = 20-50 nM
Ki Data :
endoplasmic reticulum α-1,2-mannosidase I : Ki= 130 nM (human); Golgi Class I mannosidases IA, IB and IC: Ki= 23 nM (human)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :

Download SDS (MSDS)

Certificate of Analysis

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Kifunensine (CAS 109944-15-2)  Product Citations

See how others have used Kifunensine (CAS 109944-15-2). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 20 total

PMID: # 35690611  Heard, A.|Landmann, JH.|Hansen, AR.|Papadopolou, A.|Hsu, YS.|Selli, ME.|Warrington, JM.|Lattin, J.|Chang, J.|Ha, H.|Haug-Kroeper, M.|Doray, B.|Gill, S.|Ruella, M.|Hayer, KE.|Weitzman, MD.|Green, AM.|Fluhrer, R.|Singh, N.| et al. 2022. Nat Commun. 13: 3367.

PMID: # 33671632  Manica, G.|Ghenea, S.|Munteanu, CVA.|Martin, EC.|Butnaru, C.|Surleac, M.|Chiritoiu, GN.|Alexandru, PR.|Petrescu, AJ.|Petrescu, SM.| et al. 2021. Int J Mol Sci. 22:

PMID: # 34211491  Shin, YJ.|König-Beihammer, J.|Vavra, U.|Schwestka, J.|Kienzl, NF.|Klausberger, M.|Laurent, E.|Grünwald-Gruber, C.|Vierlinger, K.|Hofner, M.|Margolin, E.|Weinhäusel, A.|Stöger, E.|Mach, L.|Strasser, R.| et al. 2021. Front Plant Sci. 12: 689104.

PMID: # 34332121  Munteanu, CVA.|Chirioiu, GN.|Chirioiu, M.|Ghenea, S.|Petrescu, AJ.|Petrescu, M.| et al. 2021. Mol Cell Proteomics. 100125.

PMID: # 30971450  Schoberer, J.|Liebminger, E.|Vavra, U.|Veit, C.|Grünwald-Gruber, C.|Altmann, F.|Botchway, SW.|Strasser, R.| et al. 2019. Plant Physiol. 180: 859-873.

PMID: # 29361539  Wilson, KM. et al. 2018. J. Cell. Sci. 131:

PMID: # 30574158  Veit, C.|König, J.|Altmann, F.|Strasser, R.| et al. 2018. Front Plant Sci. 9: 1807.

PMID: # 27754876  Park, D. et al. 2016. Mol. Cell Proteomics. 15: 3653-3664.

PMID: # 24550396  Damerow, M. et al. 2014. J. Biol. Chem. 289: 9328-39.

PMID: # 25354954  Voss, M. et al. 2014. The EMBO journal. 33: 2890-905.

Citations 1 to 10 of 20 total

How much Kifunensine to use in cell culture? I don't know how much to add into a cell culture plate with 2ml of media.

Asked by: Jz28sail
Thank you for your question. This product has not been specifically tested for cell culture use and is not cell culture grade. However, most products can still be used in cell culture applications by following basic cell culture protocols, e.g. using cell culture grade/sterilized solvents, aseptic technique, and sterile filtering in a sterile hood. I provided a couple of references below: Pubmed ID: 21795794 Pubmed ID: 2144287 If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-24
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Rated 5 out of 5 by from scientific researcher Very useful for studying ER glycosylation trimming events!
Date published: 2018-10-25
Rated 5 out of 5 by from Hosokawa Hosokawa, et. al. (PubMed ID 17727818) found that kifunensine completely inhibits mannose trimming from NHK in mock transfected cells (HEK293). It also greatly diminishes in cells that have been transfected to over-express Golgi 1,2-mannosidase. -SCBT Publication Review
Date published: 2015-06-18
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