anti-Cy5 Activators
The function of anti-Cy5, a specific antibody targeting the Cy5 dye, is critical for various applications in biological research, including fluorescence microscopy, flow cytometry, and immunoblotting. Antibodies like anti-Cy5 are designed to bind with high specificity to their antigens, in this case, the Cy5 dye, enabling the detection and quantification of target molecules tagged with this fluorescent label. The effectiveness of such detection relies on the stability of the antibody, the specificity of its binding, and the visibility of the fluorescent signal.
Chemicals that enhance the binding process or detection capability of the anti-Cy5 antibody do so by optimizing the conditions under which these antibodies operate rather than activating them in a traditional biochemical sense. Stabilizing the antibody, preventing nonspecific interactions, and enhancing the fluorescent signal's visibility are key strategies for improving the performance of anti-Cy5 in various assays. These approaches ensure that the antibody maintains its conformation and binding affinity over time, reduces background noise by minimizing nonspecific binding, and enhances the detectability of the Cy5 signal, thereby facilitating accurate and sensitive detection of Cy5-tagged targets in complex biological samples. Through these indirect means, the listed chemicals contribute to the effective use of anti-Cy5 antibodies in research and diagnostic applications, highlighting the importance of optimizing experimental conditions for the successful utilization of fluorescent antibodies.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Glycerol | 56-81-5 | sc-29095A sc-29095 | 100 ml 1 L | $55.00 $150.00 | 12 | |
Glycerol is used to stabilize proteins in solution, potentially enhancing the stability and function of anti-Cy5 antibody by maintaining its conformation, thereby facilitating better binding to Cy5. | ||||||
Sodium azide | 26628-22-8 | sc-208393 sc-208393B sc-208393C sc-208393D sc-208393A | 25 g 250 g 1 kg 2.5 kg 100 g | $42.00 $152.00 $385.00 $845.00 $88.00 | 8 | |
Sodium Azide is commonly used as a preservative in antibody solutions, preventing microbial growth that could degrade antibody quality and binding efficiency over time. | ||||||
Tween-20 | 9005-64-5 | sc-29113C sc-29113 sc-29113B sc-29113A | 100 ml 500 ml 1 L 3.8 L | $18.00 $30.00 $50.00 $160.00 | 54 | |
Tween 20 is a surfactant used in washing steps of immunoassays to reduce nonspecific binding, indirectly improving the signal-to-noise ratio in assays involving anti-Cy5. | ||||||
Tris base | 77-86-1 | sc-3715 sc-3715A sc-3715B sc-3715C | 500 g 1 kg 5 kg 10 kg | $33.00 $58.00 $251.00 $556.00 | 13 | |
TBS is used to maintain pH and osmolarity in antibody binding reactions, optimizing conditions for anti-Cy5 antibody binding to Cy5. | ||||||
Dimethyl Sulfoxide (DMSO) | 67-68-5 | sc-202581 sc-202581A sc-202581B | 100 ml 500 ml 4 L | $30.00 $115.00 $900.00 | 136 | |
DMSO can be used to dissolve small molecules that could quench the fluorescence of Cy5, potentially enhancing the visibility of the Cy5 signal detected by anti-Cy5. | ||||||
Sucrose | 57-50-1 | sc-204311 sc-204311B sc-204311C sc-204311A | 0.5 kg 50 kg 100 kg 5 kg | $57.00 $1224.00 $1760.00 $195.00 | 6 | |
Sucrose is used as a stabilizer in protein formulations, which can protect the anti-Cy5 antibody from degradation during storage and use, maintaining its binding capacity. | ||||||
Magnesium chloride | 7786-30-3 | sc-255260C sc-255260B sc-255260 sc-255260A | 10 g 25 g 100 g 500 g | $27.00 $34.00 $47.00 $123.00 | 2 | |
Magnesium chloride is used in reaction buffers to stabilize the tertiary and quaternary structures of proteins, potentially enhancing the binding affinity of anti-Cy5 for Cy5. | ||||||
Sodium Chloride | 7647-14-5 | sc-203274 sc-203274A sc-203274B sc-203274C | 500 g 2 kg 5 kg 10 kg | $18.00 $23.00 $35.00 $65.00 | 15 | |
Sodium chloride is used to adjust the ionic strength of solutions, which can influence the binding kinetics of antibody-antigen interactions, possibly optimizing anti-Cy5 binding to Cy5. | ||||||
PEG 1000 | 25322-68-3 | sc-203182 sc-203182A | 25 g 250 g | $17.00 $40.00 | 1 | |
PEG is used to precipitate proteins, which can concentrate the anti-Cy5 antibody and enhance its interaction with Cy5 by reducing the volume in which binding occurs. | ||||||
L-Arginine | 74-79-3 | sc-391657B sc-391657 sc-391657A sc-391657C sc-391657D | 5 g 25 g 100 g 500 g 1 kg | $20.00 $30.00 $60.00 $215.00 $345.00 | 2 | |
Arginine is used as an additive in protein formulations to improve solubility and stability, potentially enhancing the functional binding of anti-Cy5 to its antigen, Cy5. | ||||||