Tris base CAS: 77-86-1
MF: C4H11NO3
MW: 121.14
A high quality buffer used in the range of physiological pH 7-9.

Tris base (CAS 77-86-1)

Tris base | CAS 77-86-1 is rated 4.9 out of 5 by 10.
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Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol
Application: A high quality buffer used in the range of physiological pH 7-9
CAS Number: 77-86-1
Purity: ≥99%
Molecular Weight: 121.14
Molecular Formula: C4H11NO3
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.


1. Kallet, R.H. et al. 2000. Am. J. Respir. Crit. Care Med. 161(4 Pt 1): 1149-1153. PMID: 10764304

2. Desmarais, W.T. et al. 2002. Structure. 10(8): 1063-1072. PMID: 12176384

3. Ghalanbor, Z. et al. 2008. Protein Pept. Lett. 15(2): 212-214. PMID: 18289113

4. Sambrook, J. and Russell, D.W. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor, NY. CSHL Press.

Usage :
Stable for up to 1 year after receipt when stored at -20°C. Stock solutions should be aliquoted and stored at -20°C for up to 1 month.
Appearance :
Crystalline powder
Physical State :
Solubility :
Soluble in water (550 mg/ml), ethylene glycol (79.1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), 95% ethanol (22 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0.5 mg/ml), olive oil (0.4 mg/ml), chloroform (0.05 mg/ml), and alkaline solution.
pH :
useful pH range: 7.0 - 9.0
Storage :
Store at room temperature
Melting Point :
168-172° C
Boiling Point :
219-220° C at 10 mmHg
Density :
~1.3 g/cm3
Refractive Index :
n20D 1.54 (Predicted)
pK Values :
pKa: 8.2 (at 20° C), 8.06 (at 25° C), 7.72 (at 37° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :
Merck Index :
14: 9772
MDL Number :
EC Number :
Beilstein Registry :

Download SDS (MSDS)

Certificate of Analysis

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Tris base  Product Citations

See how others have used Tris base. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 31123703  Di Rienzo, M.|Antonioli, M.|Fusco, C.|Liu, Y.|Mari, M.|Orhon, I.|Refolo, G.|Germani, F.|Corazzari, M.|Romagnoli, A.|Ciccosanti, F.|Mandriani, B.|Pellico, MT.|De La Torre, R.|Ding, H.|Dentice, M.|Neri, M.|Ferlini, A.|Reggiori, F.|Kulesz-Martin, M.|Piacentini, M.|Merla, G.|Fimia, GM.| et al. 2019. Sci Adv. 5: eaau8857.

PMID: # 26534833  Khnouf, R. et al. 2016. Electrophoresis. 37: 529-35.

PMID: # 28573163  Fiesel, FC. et al. 2016. Bio Protoc. 6:

PMID: # 12124396  Hansson, LO. et al. 2002. Proc. Natl. Acad. Sci. U.S.A. 99: 10305-10309.

Citations 1 to 4 of 4 total

je voudrais consulter le fiche de données de sécurité de TRIS

Asked by: MN23
Merci pour votre question. La MSDS est disponible à télécharger directement en ligne sur la page web du produit, dans la partie "SDS et Certificat d'Analyse". Veuillez contacter notre Service Technique pour toute information complémentaire sur ce produit. Thank you for your question. You can download the MSDS for this product on its product page under 'SDS & Certificate of Analysis'. MSDS documentation for other articles from our ChemCruz product line can be downloaded on the individual product pages. If you have further questions, please contact our technical service directly.
Answered by: Technical Support Europe
Date published: 2019-02-19

interested in a price offer for: Tris base, 500 g sc-3715 500 g, CAS: 77-86-1

Asked by: Adva
Thank you for your question. Please contact one of our authorized distributors for information about current product availability and shipping lead times.
Answered by: Technical Support
Date published: 2019-01-15

do you sell TrisBase-HCL? How much does it cost? Thanks

Asked by: LENny
Thank you for your question. Yes, we offer several different TrisBase-HCL. You can find them here:
Answered by: Tech Service
Date published: 2019-01-14

What is the country of origin?

Asked by: SCM4
Previous lots have originated in China.
Answered by: Tech Service 11
Date published: 2017-03-17

Is this compound molecular biology grade?

Asked by: SCchem16
Thank you for your question. This chemical, Tris base (CAS 77-86-1), Is not tested for Molecular biology grade. However, it is high quality and we suggest using Molecular biology grade solvents for filtering and testing a sample prior to use.
Answered by: Chemical Support 2
Date published: 2019-12-10
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Rated 5 out of 5 by from Excellent Customer Care Always a pleasure to do business with SCTB. Great customer & technical service, timely shipment and quality chemicals.
Date published: 2019-06-28
Rated 5 out of 5 by from Tris-base I bought this product a month ago and it is Excellent product!
Date published: 2019-05-15
Rated 5 out of 5 by from Great quality TRIS We use Tris base from SCBT for a few years for all kinds of buffers and especially for wet transfer buffer and TBS (tris buffer saline) for antibody incubation solutions. Works great every time!
Date published: 2019-01-08
Rated 5 out of 5 by from good product and fast shipping! I'll buy more stuff from your company. Your sale persons are very nice and professional.
Date published: 2018-09-05
Rated 5 out of 5 by from Great and cheap! It can be used for our electrophoresis buffer. No difference from other vendors.
Date published: 2017-12-29
Rated 5 out of 5 by from Nice product It is much cheaper than Sigma. Strongly recommend it!
Date published: 2017-09-27
Rated 5 out of 5 by from Great value. We bought this month ago and we are perfectly happy with that.
Date published: 2017-03-23
Rated 5 out of 5 by from great quality and cheaper I bought this product and It is much cheaper compared to others!
Date published: 2016-11-29
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