ANKRD27 Activators
ANKRD27 engage in various molecular interactions to initiate its activation. Phorbol 12-myristate 13-acetate (PMA), by activating protein kinase C (PKC), triggers the phosphorylation of ANKRD27, which enhances its interactions with other proteins and influences its subcellular localization, pivotal for vesicle trafficking. Forskolin, by increasing intracellular cAMP levels, indirectly activates protein kinase A (PKA), which may also phosphorylate and thereby activate ANKRD27. Similar effects on PKA are achieved by dibutyryl cAMP and 8-Bromo-cAMP, both cAMP analogs that permeate cells and activate PKA, leading to possible phosphorylation events involving ANKRD27.
Ionomycin and A23187 (Calcimycin) elevate intracellular calcium levels, thus activating calcium-dependent proteins that may interact with ANKRD27, influencing its role in endosomal sorting and trafficking. Thapsigargin contributes to this process by inhibiting the SERCA pump, resulting in increased cytosolic calcium and subsequent activation of calcium-binding proteins that may modulate ANKRD27. Calyculin A and okadaic acid, as inhibitors of protein phosphatases 1 and 2A, maintain the phosphorylation state of proteins, including ANKRD27, by preventing dephosphorylation, thus fostering an environment conducive to ANKRD27 activity. Phosphatidic acid, as a lipid signaling molecule, might trigger signaling cascades with potential effects on ANKRD27's activation by modifying its interactions or localization. Although BIM-1 is a PKC inhibitor, it may indirectly influence the cellular signaling milieu, creating conditions that favor ANKRD27 activation. Lastly, epigallocatechin gallate (EGCG) inhibits phosphodiesterases, which increases cAMP levels, thereby activating PKA, which in turn can lead to phosphorylation and subsequent activation of ANKRD27.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which can phosphorylate ANKRD27. This phosphorylation can lead to functional activation of ANKRD27 by enhancing its interactions with other proteins or by affecting its subcellular localization, which is crucial for its role in vesicle trafficking. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylyl cyclase, increasing cAMP levels, which in turn activates PKA. PKA can phosphorylate various substrates that may include ANKRD27, potentially leading to its activation and regulation of its role in membrane trafficking. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calcium-dependent proteins that may interact with or modify ANKRD27, leading to its activation and facilitation of its function in endosomal sorting and trafficking. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000C | 10 µg 100 µg 1 mg | $160.00 $750.00 $3000.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A). Inhibition of these phosphatases can result in increased phosphorylation levels of proteins, including ANKRD27. This would lead to an increase in ANKRD27 activity as part of its role in mediating vesicle transport. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Similar to calyculin A, okadaic acid is a potent inhibitor of PP1 and PP2A, leading to hyperphosphorylation of proteins. This can contribute to the activation of ANKRD27 by maintaining its phosphorylation state, which is necessary for its function in vesicular trafficking pathways. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl cAMP is a cell-permeable analog of cAMP that activates PKA. Activation of PKA can lead to phosphorylation of substrates that interact with or regulate ANKRD27, thereby promoting its activation and its function in the regulation of intracellular transport processes. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $97.00 $224.00 | 30 | |
8-Bromo-cAMP is another cell-permeable cAMP analog that activates PKA. The activation of PKA can result in phosphorylation and consequential activation of ANKRD27, enhancing its role in vesicle formation and trafficking. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor that leads to increased cytosolic calcium levels. The rise in intracellular calcium can activate calmodulin and other calcium-binding proteins, which might directly interact with or modify ANKRD27, resulting in its activation and participation in endosomal sorting. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is a calcium ionophore like ionomycin, leading to an increase in intracellular calcium concentration. The elevated calcium can activate calcium-dependent proteins that modulate ANKRD27 function, potentially leading to its activation and involvement in trafficking and signaling endosomes. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG is known to modulate various signaling pathways and can inhibit phosphodiesterases, leading to increased cAMP levels in the cell. This can activate PKA and lead to subsequent phosphorylation and activation of ANKRD27, which is involved in vesicular transport processes. | ||||||