ADAT2 Inhibitors
Chemical inhibitors of ADAT2 can be chosen based on their ability to disrupt cellular processes that are essential for the protein's function. Methylene Blue, by inhibiting nitric oxide synthase, can decrease the levels of nitric oxide within cells, which in turn may affect RNA metabolism and indirectly inhibit ADAT2's role in tRNA modification. Lead acetate might inhibit ADAT2 by binding to its essential metal ions, while sodium arsenite can inhibit enzymes with critical cysteine residues, which, if present in ADAT2, would impair its function. Chloroquine alters the pH of intracellular compartments such as lysosomes, potentially disrupting ADAT2's trafficking or degradation. Brefeldin A targets protein trafficking between the endoplasmic reticulum and the Golgi, and if ADAT2 requires this route for proper folding or maturation, its function would be compromised.
On the other hand, 3-Methyladenine inhibits autophagy, a process that could degrade ADAT2 under certain conditions, leading to the accumulation of possibly non-functional forms of the protein. MG132 blocks proteasomal degradation, a pathway that might regulate ADAT2 turnover, resulting in the buildup of defective ADAT2 protein. Compounds like cycloheximide and puromycin interrupt protein synthesis, which would prevent the replenishment of ADAT2 levels in the cell, thereby indirectly inhibiting its activity. Actinomycin D and α-Amanitin inhibit RNA synthesis, which could reduce the availability of tRNA substrates necessary for ADAT2's modification functions, essentially limiting its activity by depriving it of its substrates. Lastly, camptothecin disrupts DNA replication processes, leading to a cellular environment that is less conducive to protein synthesis, including the synthesis of ADAT2, therefore lowering the functional presence of ADAT2 in the cell.
SEE ALSO...
Items 1 to 10 of 12 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Methylene blue | 61-73-4 | sc-215381B sc-215381 sc-215381A | 25 g 100 g 500 g | $43.00 $104.00 $328.00 | 3 | |
Methylene Blue can act as an inhibitor of nitric oxide synthase (NOS). Since ADAT2 is involved in tRNA modification, and nitric oxide has been reported to influence various aspects of RNA metabolism and function, the inhibition of NOS can reduce nitric oxide levels, which in turn could decrease the modulation of tRNA functions and indirectly inhibit the ADAT2 protein's activity in these processes. | ||||||
Lead(II) Acetate | 301-04-2 | sc-507473 | 5 g | $85.00 | ||
Lead acetate is a compound that can inhibit various enzymes by displacing essential metal ions in their active sites. If ADAT2 requires metal ions for its catalytic activity, lead acetate could bind to these sites and inhibit the enzyme's function. | ||||||
Sodium (meta)arsenite | 7784-46-5 | sc-250986 sc-250986A | 100 g 1 kg | $108.00 $780.00 | 3 | |
Sodium arsenite can bind to vicinal thiols and potentially inhibit enzymes that rely on cysteine residues for activity. If ADAT2 has essential cysteine residues at its active site or for maintaining structural integrity, sodium arsenite could inhibit its activity by binding to these residues. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $69.00 | 2 | |
Chloroquine is known to alkalinize acidic compartments within cells, such as lysosomes. If the activity of ADAT2 is dependent on acidic cellular compartments or if ADAT2 is degraded in lysosomes, chloroquine could indirectly inhibit ADAT2 by disrupting its normal intracellular trafficking or degradation. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $31.00 $53.00 $124.00 $374.00 | 25 | |
Brefeldin A disrupts protein trafficking by inhibiting protein transport from the endoplasmic reticulum to the Golgi apparatus. If ADAT2 requires post-translational modifications or trafficking through the Golgi for its activity, Brefeldin A could inhibit its function. | ||||||
Autophagy Inhibitor, 3-MA | 5142-23-4 | sc-205596 sc-205596A | 50 mg 500 mg | $65.00 $261.00 | 113 | |
3-Methyladenine is an autophagy inhibitor that can block the formation of autophagosomes. If ADAT2 is degraded through autophagy, inhibition of this pathway could increase ADAT2 protein levels, leading to a functional inhibition due to a negative feedback mechanism or accumulation of non-functional protein. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $60.00 $265.00 $1000.00 | 163 | |
MG132 is a proteasome inhibitor. If ADAT2 is regulated through proteasomal degradation, inhibiting this pathway could lead to the accumulation of misfolded or damaged ADAT2 protein, which could be functionally inactive. | ||||||
Cycloheximide | 66-81-9 | sc-3508B sc-3508 sc-3508A | 100 mg 1 g 5 g | $41.00 $84.00 $275.00 | 127 | |
Cycloheximide inhibits protein synthesis by interfering with the translocation step in protein synthesis, hence preventing the elongation of the polypeptide chain. If ADAT2 has a high turnover rate, the inhibition of protein synthesis could decrease its functional presence in the cell. | ||||||
Puromycin dihydrochloride | 58-58-2 | sc-108071 sc-108071B sc-108071C sc-108071A | 25 mg 250 mg 1 g 50 mg | $42.00 $214.00 $832.00 $66.00 | 394 | |
Puromycin causes premature chain termination during protein synthesis by acting as an analog of aminoacyl-tRNA. If ADAT2 is rapidly turned over and requires constant synthesis, puromycin could inhibit the accumulation of functional ADAT2 protein. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $74.00 $243.00 $731.00 $2572.00 $21848.00 | 53 | |
Actinomycin D binds to DNA and inhibits RNA synthesis. While it does not inhibit ADAT2 transcription, it could reduce the overall levels of tRNA substrates available for ADAT2, thus functionally inhibiting its activity by limiting its ability to modify tRNAs. | ||||||