1810015C04Rik Activators
The term 1810015C04Rik Activators pertains to a specific set of chemicals that are identified for their role in activating the gene encoded by the 1810015C04Rik locus. This gene is known to be involved in various cellular pathways and functions. The process of discovering activators for this gene begins with high-throughput screening campaigns, where libraries of thousands of diverse chemical compounds are tested for their ability to modulate gene expression. For the purpose of detection, the gene is often linked to a reporter system, such as the luciferase reporter gene. When a compound interacts with the gene's promoter region and activates it, the luciferase gene is expressed, emitting a quantifiable luminescent signal. The brightness of this signal correlates directly with the level of gene activation. Compounds that lead to a substantial increase in luminescence are selected for further investigation, as they are indicative of successful gene activation.
After the initial identification of potential activators, further validation is pursued through a series of in-depth analyses. Quantitative PCR (qPCR) is one such method, providing a precise measurement of mRNA levels for the 1810015C04Rik gene. An observed increase in mRNA concentration indicates that transcription is being enhanced by the activator compounds. To confirm that these transcriptional changes translate to an increase in protein levels, Western blot analysis is conducted. This technique allows for the detection and quantification of the 1810015C04Rik protein, offering insights into the protein's expression profile in the presence of the activating compounds. Moreover, it can reveal information about the protein's stability and any post-translational modifications that may result from the activation process.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. This rise in calcium can activate calcium-dependent protein kinases, which may then phosphorylate and activate "1810015C04Rik Protein" if it is regulated by calcium-dependent processes. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which may phosphorylate "1810015C04Rik Protein" or its associated proteins, potentially enhancing its activity if it is part of the PKC signaling pathway. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG is a tyrosine kinase inhibitor that could reduce competitive signaling pathways, potentially allowing "1810015C04Rik Protein" to be more active if it operates downstream of tyrosine kinase pathways. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a PI3K inhibitor that can modulate the PI3K/Akt pathway. Inhibition of this pathway may reduce negative feedback loops, potentially enhancing the activity of "1810015C04Rik Protein" if it is positively regulated by the PI3K pathway. | ||||||
D-erythro-Sphingosine-1-phosphate | 26993-30-6 | sc-201383 sc-201383D sc-201383A sc-201383B sc-201383C | 1 mg 2 mg 5 mg 10 mg 25 mg | $162.00 $316.00 $559.00 $889.00 $1693.00 | 7 | |
Sphingosine-1-phosphate engages S1P receptors, which can activate signaling cascades that may include "1810015C04Rik Protein" if it is involved in sphingolipid signaling pathways. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca^2+ ATPase (SERCA), leading to increased cytosolic calcium levels that can activate calcium-dependent signaling pathways, possibly enhancing the activity of "1810015C04Rik Protein". | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $88.00 $342.00 | 284 | |
SB203580 is a p38 MAPK inhibitor that may shift signaling towards pathways in which "1810015C04Rik Protein" is active, thus enhancing its activity if it is involved in p38 MAPK regulated processes. | ||||||
Genistein | 446-72-0 | sc-3515 sc-3515A sc-3515B sc-3515C sc-3515D sc-3515E sc-3515F | 100 mg 500 mg 1 g 5 g 10 g 25 g 100 g | $26.00 $92.00 $120.00 $310.00 $500.00 $908.00 $1821.00 | 46 | |
Genistein is a tyrosine kinase inhibitor that can increase the activity of pathways or proteins not directly related to tyrosine kinase signaling, potentially enhancing "1810015C04Rik Protein" activity if it is associated with such pathways. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $150.00 $388.00 | 113 | |
Staurosporine is a broad-spectrum protein kinase inhibitor, which, despite its general inhibition, could selectively activate "1810015C04Rik Protein" by specifically inhibiting kinases that negatively regulate it. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is a calcium ionophore that facilitates the increase of intracellular calcium levels, which may activate "1810015C04Rik Protein" if it is regulated by calcium-dependent signaling. | ||||||