Blocking (neutralizing) peptides are available as negative controls for all Santa Cruz Biotechnology, Inc. affinity-purified antibodies raised against peptide antigens. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide.
Determine the highest antibody dilution at which a consistently positive result is achieved for the desired test. For example, H-Ras (259) is recommended for immunoprecipitation at 1 µg/ml but is positive at a dilution of 50 ng/ml.
For blocking/competition, combine antibody (at a concentration determined by the aforementioned method) with a five-fold (by weight) excess of blocking peptide in a small volume (500 µl) of PBS (Buffers and General Solutions). Incubate for up to 2 hours at room temperature or overnight at 4° C.
Following blocking/competition, dilute antibody/peptide mixture into appropriate blocking buffer and proceed with the desired research application.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
Create a new account
Email address already exists, please enter a new valid email address.Hide
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.