H-Ras Antibody (259): sc-35
- H-Ras Antibody (259) is a rat monoclonal IgG1 H-Ras antibody, cited in 184 publications, provided at 200 µg/ml
- Anti-H-Ras Antibody (259) is recommended for detection of antigenic determinants common to H-, K- and N-Ras p21 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
- H-Ras Antibody (259) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- available azide-free for blocking H-Ras binding of Raf (sc-35 L, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for H-Ras Antibody (259). This work is in progress.
H-Ras Antibody (259) is a rat monoclonal IgG1 that detects H-Ras in mouse, rat, and human samples through various techniques including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). H-Ras is a small GTPase that plays a crucial role in regulating signal transduction pathways related to cell growth, differentiation, and apoptosis. The subcellular localization of H-Ras is particularly important for its function. H-Ras primarily resides at the plasma membrane, where H-Ras is activated to initiate signaling cascades. H-Ras can also localize to the endoplasmic reticulum and Golgi apparatus, where H-Ras can engage different signaling pathways, affecting processes such as proliferation and transformation. The correct localization is achieved through post-translational modifications, including palmitoylation and farnesylation, which facilitate membrane attachment. Disruption of this localization, for example, by inhibiting the palmitoylation process, can impair H-Ras signaling, underscoring H-Ras dependence on membrane association for effective function. H-Ras (259) monoclonal antibody is available in both non-conjugated and multiple conjugated forms, making H-Ras (259) antibody versatile for various experimental applications, including the detection and study of H-Ras in different cellular compartments and under different experimental conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
H-Ras Antibody (259) References:
- GTP-dependent segregation of H-ras from lipid rafts is required for biological activity. | Prior, IA., et al. 2001. Nat Cell Biol. 3: 368-75. PMID: 11283610
- H-Ras signaling and K-Ras signaling are differentially dependent on endocytosis. | Roy, S., et al. 2002. Mol Cell Biol. 22: 5128-40. PMID: 12077341
- Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors. | Arozarena, I., et al. 2004. Mol Cell Biol. 24: 1516-30. PMID: 14749369
- Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane. | Rotblat, B., et al. 2004. Mol Cell Biol. 24: 6799-810. PMID: 15254246
- Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signaling. | Roy, S., et al. 2005. Mol Cell Biol. 25: 6722-33. PMID: 16024806
- H-Ras dynamically interacts with recycling endosomes in CHO-K1 cells: involvement of Rab5 and Rab11 in the trafficking of H-Ras to this pericentriolar endocytic compartment. | Gomez, GA. and Daniotti, JL. 2005. J Biol Chem. 280: 34997-5010. PMID: 16079139
- A novel switch region regulates H-ras membrane orientation and signal output. | Abankwa, D., et al. 2008. EMBO J. 27: 727-35. PMID: 18273062
- Nonsteroidal anti-inflammatory drugs alter the spatiotemporal organization of Ras proteins on the plasma membrane. | Zhou, Y., et al. 2012. J Biol Chem. 287: 16586-95. PMID: 22433858
- H-ras distribution and signaling in plasma membrane microdomains are regulated by acylation and deacylation events. | Agudo-Ibáñez, L., et al. 2015. Mol Cell Biol. 35: 1898-914. PMID: 25776558
- Individual S-acylated cysteines differentially contribute to H-Ras endomembrane trafficking and acylation/deacylation cycles. | Pedro, MP., et al. 2017. Mol Biol Cell. 28: 962-974. PMID: 28179458
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H-Ras Antibody (259) | sc-35 | 200 µg/ml | $322.00 | |||
H-Ras Antibody (259) L | sc-35 L | 200 µg/0.1 ml | $322.00 | |||
H-Ras Antibody (259) AC | sc-35 AC | 500 µg/ml, 25% agarose | $424.00 | |||
H-Ras Antibody (259) HRP | sc-35 HRP | 200 µg/ml | $322.00 | |||
H-Ras Antibody (259) FITC | sc-35 FITC | 200 µg/ml | $336.00 | |||
H-Ras Antibody (259) PE | sc-35 PE | 200 µg/ml | $349.00 | |||
H-Ras Antibody (259) Alexa Fluor® 488 | sc-35 AF488 | 200 µg/ml | $364.00 | |||
H-Ras Antibody (259) Alexa Fluor® 546 | sc-35 AF546 | 200 µg/ml | $364.00 | |||
H-Ras Antibody (259) Alexa Fluor® 594 | sc-35 AF594 | 200 µg/ml | $364.00 | |||
H-Ras Antibody (259) Alexa Fluor® 647 | sc-35 AF647 | 200 µg/ml | $364.00 | |||
H-Ras Antibody (259) Alexa Fluor® 680 | sc-35 AF680 | 200 µg/ml | $364.00 | |||
H-Ras Antibody (259) Alexa Fluor® 790 | sc-35 AF790 | 200 µg/ml | $364.00 | |||
H-Ras (259) Neutralizing Peptide | sc-35 P | 100 µg/0.5 ml | $69.00 |