pan Ras Antibody (F132): sc-32

pan Ras Antibody (F132) is a mouse monoclonal IgG2b kappa light chain antibody that detects pan Ras, which includes antigenic determinants common to H-Ras, K-Ras, and N-Ras p21, in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-pan Ras antibody (F132) is available in a non-conjugated form, allowing for versatile applications in various experimental settings. The Ras proteins, encoded by the c-H-, c-K-, and N-Ras proto-oncogenes, are critical guanine nucleotide-binding proteins that play a pivotal role in cell signaling pathways, influencing cell growth, differentiation, and survival. These proteins are predominantly located at the inner surface of the plasma membrane, where they interact with various effectors to propagate signals from growth factor receptors. The membrane localization is essential for their function, as Ras proteins engage with downstream signaling pathways that regulate cellular responses to external stimuli. Dysregulation of Ras signaling, often due to mutations in the Ras genes, is implicated in numerous cancers, making the study of these proteins vital for understanding tumorigenesis and developing targeted therapies. Ras proteins interact with several other proteins, including guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), which further modulate their activity and influence cellular outcomes.

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pan Ras Antibody (F132) References:

1. Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin.  |  Pan, F., et al. 2007. Nature. 445: 433-6. PMID: 17230191
2. Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21rap1.  |  Rubinfeld, B., et al. 1991. Cell. 65: 1033-42. PMID: 1904317
3. The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21.  |  Martin, GA., et al. 1990. Cell. 63: 843-9. PMID: 2121370
4. A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants.  |  Trahey, M. and McCormick, F. 1987. Science. 238: 542-5. PMID: 2821624
5. The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene product.  |  Calés, C., et al. 1988. Nature. 332: 548-51. PMID: 2833702
6. Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain.  |  Adari, H., et al. 1988. Science. 240: 518-21. PMID: 2833817
7. ras genes.  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
8. Guanine nucleotide-binding and autophosphorylating activities associated with the p21src protein of Harvey murine sarcoma virus.  |  Shih, TY., et al. 1980. Nature. 287: 686-91. PMID: 6253810
9. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes.  |  Ellis, RW., et al. 1981. Nature. 292: 506-11. PMID: 6265801

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pan Ras Antibody (F132) has 70 citations in a variety of scientific publications.

Primary antibodies like Anti-pan Ras Antibody (F132) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pan Ras Antibody (F132) Specifications

Alternate Product Name: pan Ras

Product Clone Name: F132

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: pan Ras

Epitope Species:

Product Reactivity/Detection: antigenic determinants common to H-Ras, K-Ras and N-Ras p21

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 70 citations in various scientific publications

Available Conjugates/Forms for pan Ras Antibody (F132): N/A