Thrombin R Antibody (G-7): sc-133128

Thrombin R Antibody (G-7) is a mouse monoclonal IgG2a antibody that detects Thrombin R in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Thrombin R, also known as protease-activated receptor-1 (PAR-1), plays a crucial role in mediating the effects of thrombin, a key serine protease involved in blood coagulation and platelet aggregation. Thrombin R (G-7) antibody helps researchers study PAR-1′s ability to activate intracellular signaling pathways upon thrombin binding, which leads to physiological responses such as platelet activation and vascular remodeling. PAR-1 is primarily located on the surface of platelets and endothelial cells, where PAR-1 undergoes a unique process of internalization and degradation following activation. This internalization is essential for regulating signaling capacity and preventing excessive activation, which could lead to pathological conditions such as thrombosis. Additionally, PAR-1 interacts with other members of the G protein-coupled receptor family, including PAR-2 and PAR-3, highlighting PAR-1′s role in a complex network of signaling pathways that govern hemostasis and inflammation. Anti-Thrombin R antibody (G-7) serves as an invaluable tool for researchers studying these critical processes in human health and disease.

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Thrombin R Antibody (G-7) References:

1. Thrombin receptor antagonists; recent advances in PAR-1 antagonist development.  |  Anderluh, M. and Dolenc, MS. 2002. Curr Med Chem. 9: 1229-50. PMID: 12052165
2. Discovery of potent peptide-mimetic antagonists for the human thrombin receptor, protease-activated receptor-1 (PAR-1).  |  Maryanoff, BE., et al. 2003. Curr Med Chem Cardiovasc Hematol Agents. 1: 13-36. PMID: 15317288
3. Non-peptidic small-molecule antagonists of the human platelet thrombin receptor PAR-1.  |  Selnick, HG., et al. 2003. Curr Med Chem Cardiovasc Hematol Agents. 1: 47-59. PMID: 15317290
4. Prothrombin/thrombin and the thrombin receptors PAR-1 and PAR-4 in the brain: localization, expression and participation in neurodegenerative diseases.  |  Sokolova, E. and Reiser, G. 2008. Thromb Haemost. 100: 576-81. PMID: 18841278
5. Tissue factor-thrombin-PAR-1 pathway: a novel link between bone marrow and blood vessel.  |  Delacroix, S. and Simari, RD. 2012. Arterioscler Thromb Vasc Biol. 32: 3-4. PMID: 22173975
6. PAR-1 mediates the thrombin-induced mesothelial cell overproduction of VEGF and PAI-1.  |  Belling, F., et al. 2013. Int J Artif Organs. 36: 97-104. PMID: 23280079
7. Investigation of thrombin activity with PAR 1-based fluorogenic peptides.  |  Vieira, SM., et al. 2013. Protein Pept Lett. 20: 1129-35. PMID: 23688151
8. Regulation of Cell Polarity by PAR-1/MARK Kinase.  |  Wu, Y. and Griffin, EE. 2017. Curr Top Dev Biol. 123: 365-397. PMID: 28236972
9. Thrombin Upregulates PAI-1 and Mesothelial-Mesenchymal Transition Through PAR-1 and Contributes to Tuberculous Pleural Fibrosis.  |  Hsieh, CY., et al. 2019. Int J Mol Sci. 20: PMID: 31614900
10. Effects of intermittent hypoxia with thrombin in an in vitro model of human brain endothelial cells and their impact on PAR-1/PAR-3 cleavage.  |  Zolotoff, C., et al. 2022. Sci Rep. 12: 12305. PMID: 35853902

The preferred Thrombin R antibody is Thrombin R Antibody (ATAP2): sc-13503.