caspase-8 p18 Antibody (D-7): sc-393776

Caspase-8 p18 Antibody (D-7) is a mouse monoclonal IgG2a antibody that detects caspase-8 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Caspase-8 plays a crucial role in the apoptotic signaling pathway, acting as an initiator caspase that activates downstream effector caspases, thereby regulating programmed cell death. Caspase-8 is primarily located in the cytoplasm and is essential for the formation of the death-inducing signaling complex (DISC) when binding to the adaptor molecule FADD. Proper localization of caspase-8 ensures efficient activation in response to apoptotic signals, particularly those mediated by tumor necrosis factor receptors. Dysregulation of caspase-8 can lead to severe consequences, including immune deficiencies and increased susceptibility to various diseases, highlighting caspase-8′s importance in maintaining cellular homeostasis and immune responses. Anti-caspase-8 antibody (D-7) serves as an invaluable tool for researchers studying apoptosis and related pathways, providing insights into the mechanisms of cell death and potential therapeutic targets for diseases associated with caspase dysfunction.

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caspase-8 p18 Antibody (D-7) References:

1. Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction.  |  Wesselborg, S., et al. 1999. Blood. 93: 3053-63. PMID: 10216102
2. The atomic-resolution structure of human caspase-8, a key activator of apoptosis.  |  Watt, W., et al. 1999. Structure. 7: 1135-43. PMID: 10508785
3. [W206R]-procaspase 3: an inactivatable substrate for caspase 8.  |  Rank, KB., et al. 2001. Protein Expr Purif. 22: 258-66. PMID: 11437602
4. Proteasome inhibition blocks caspase-8 degradation and sensitizes prostate cancer cells to death receptor-mediated apoptosis.  |  Thorpe, JA., et al. 2008. Prostate. 68: 200-9. PMID: 18076022
5. MUC1 oncoprotein blocks death receptor-mediated apoptosis by inhibiting recruitment of caspase-8.  |  Agata, N., et al. 2008. Cancer Res. 68: 6136-44. PMID: 18676836
6. Voltage dependent anion channel-1 regulates death receptor mediated apoptosis by enabling cleavage of caspase-8.  |  Chacko, AD., et al. 2010. BMC Cancer. 10: 380. PMID: 20646307
7. Vps41, a protein involved in lysosomal trafficking, interacts with caspase-8.  |  Wang, L., et al. 2013. Acta Biochim Pol. 60: 37-42. PMID: 23173123
8. Proteasomal regulation of caspase-8 in cancer cell apoptosis.  |  Fiandalo, MV., et al. 2013. Apoptosis. 18: 766-76. PMID: 23456622
9. p62/SQSTM1 is involved in caspase-8 associated cell death induced by proteasome inhibitor MG132 in U87MG cells.  |  Zeng, RX., et al. 2014. Cell Biol Int. 38: 1221-6. PMID: 24805111
10. Direct cleavage of caspase-8 by herpes simplex virus 1 tegument protein US11.  |  Musarra-Pizzo, M., et al. 2022. Sci Rep. 12: 12317. PMID: 35853963

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.