ZPBP1 Antibody (F-12): sc-393152

ZPBP1 Antibody (F-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects ZPBP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZPBP1 Antibody (F-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZPBP1, or Zona pellucida-binding protein 1, consists of 351 amino acids and is a member of the zona pellucida-binding protein Sp38 family, playing a crucial role in gamete interaction during fertilization. ZPBP1 is secreted and is essential for the binding of sperm to the zona pellucida, the outer layer of the egg, which is vital for successful fertilization and subsequent embryonic development. ZPBP1 is located on Chromosome 7, a chromosome that spans approximately 158 million bases and encodes over 1,000 genes, accounting for about 5% of the human genome. Chromosome 7 has been associated with several genetic disorders, including Osteogenesis imperfecta and Pendred syndrome, highlighting the importance of understanding ZPBP1 in relation to human health and disease.

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ZPBP1 Antibody (F-12) References:

1. The extracellular protein coat of the inner acrosomal membrane is involved in zona pellucida binding and penetration during fertilization: characterization of its most prominent polypeptide (IAM38).  |  Yu, Y., et al. 2006. Dev Biol. 290: 32-43. PMID: 16386726
2. Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis.  |  Lin, YN., et al. 2007. Mol Cell Biol. 27: 6794-805. PMID: 17664285
3. The origin and assembly of a zona pellucida binding protein, IAM38, during spermiogenesis.  |  Yu, Y., et al. 2009. Microsc Res Tech. 72: 558-65. PMID: 19204925
4. Molecular cloning of pig ZPBP2 and mRNA expression of ZPBP1 and ZPBP2 in reproductive tracts of boars.  |  Song, C., et al. 2010. Anim Reprod Sci. 122: 229-35. PMID: 20846800
5. Biochemical and binding characteristics of boar epididymal fluid proteins.  |  Maňásková-Postlerová, P., et al. 2011. J Chromatogr B Analyt Technol Biomed Life Sci. 879: 100-6. PMID: 21163710
6. The molecular basis of mouse sperm-zona pellucida binding: a still unresolved issue in developmental biology.  |  Clark, GF. 2011. Reproduction. 142: 377-81. PMID: 21730109
7. Association of mutations in the zona pellucida binding protein 1 (ZPBP1) gene with abnormal sperm head morphology in infertile men.  |  Yatsenko, AN., et al. 2012. Mol Hum Reprod. 18: 14-21. PMID: 21911476
8. Proteomic Analysis of Dpy19l2-Deficient Human Globozoospermia Reveals Multiple Molecular Defects.  |  Guo, Y., et al. 2019. Proteomics Clin Appl. 13: e1900007. PMID: 31424156
9. Fluoride exposure arrests the acrosome formation during spermatogenesis via down-regulated Zpbp1, Spaca1 and Dpy19l2 expression in rat testes.  |  Jiang, S., et al. 2019. Chemosphere. 226: 874-882. PMID: 31509916
10. [Advances in the studies of teratospermia-related genes].  |  Ma, S., et al. 2018. Zhonghua Nan Ke Xue. 24: 937-940. PMID: 32212452
11. CFAP65 is required in the acrosome biogenesis and mitochondrial sheath assembly during spermiogenesis.  |  Wang, W., et al. 2021. Hum Mol Genet. 30: 2240-2254. PMID: 34231842
12. Genetic etiological spectrum of sperm morphological abnormalities.  |  Arora, M., et al. 2024. J Assist Reprod Genet. 41: 2877-2929. PMID: 39417902

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZPBP1 Antibody (F-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ZPBP1 Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZPBP1 Antibody (F-12) Specifications

Alternate Product Name: ZPBP1

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 309-338

Epitope Target: ZPBP1

Epitope Species: human

Product Reactivity/Detection: ZPBP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ZPBP1 Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin