ZO-2 Antibody (E-5): sc-514557

ZO-2 Antibody (E-5) is a mouse monoclonal IgG1 antibody that detects ZO-2 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZO-2 is a crucial component of tight junctions, which are specialized structures that maintain the integrity of epithelial and endothelial cell layers by regulating paracellular permeability. ZO-2 is primarily located at the cell membrane, where anti-ZO-2 antibody (E-5) reveals its vital role in establishing and maintaining cell polarity and barrier function. ZO-2 interacts with other tight junction proteins, including ZO-1 and claudins, to form a complex that not only provides structural support but also facilitates signaling pathways essential for cellular communication and homeostasis. Alterations in ZO-2 expression have been implicated in various pathological conditions, including cancer, where disrupted tight junction integrity can lead to increased invasiveness and metastasis. ZO-2 monoclonal antibody (E-5) serves as an invaluable tool for researchers investigating the dynamics of tight junctions and their role in health and disease.

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ZO-2 Antibody (E-5) References:

1. ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation.  |  Umeda, K., et al. 2006. Cell. 126: 741-54. PMID: 16923393
2. Altered expression of ZO-1 and ZO-2 in Sertoli cells and loss of blood-testis barrier integrity in testicular carcinoma in situ.  |  Fink, C., et al. 2006. Neoplasia. 8: 1019-27. PMID: 17217619
3. Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function.  |  Tsukita, S., et al. 2009. Ann N Y Acad Sci. 1165: 44-52. PMID: 19538286
4. ZO-2 Suppresses Cell Migration Mediated by a Reduction in Matrix Metalloproteinase 2 in Claudin-18-Expressing Lung Adenocarcinoma A549 Cells.  |  Akizuki, R., et al. 2019. Biol Pharm Bull. 42: 247-254. PMID: 30713254
5. ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver.  |  Xu, J., et al. 2022. NPJ Regen Med. 7: 55. PMID: 36151109
6. Polyubiquitination and SUMOylation Sites Regulate the Stability of ZO-2 Protein and the Sealing of Tight Junctions.  |  Cano-Cortina, M., et al. 2022. Cells. 11: PMID: 36291162
7. Transcriptomic Analysis of Tight Junction Proteins Demonstrates the Aberrant Expression and Function of Zona Occludens 2 (ZO-2) Protein in Stanford Type A Aortic Dissection.  |  Magouliotis, DE., et al. 2023. J Pers Med. 13: PMID: 38138924
8. The Role of ZO-2 in Modulating JAM-A and γ-Actin Junctional Recruitment, Apical Membrane and Tight Junction Tension, and Cell Response to Substrate Stiffness and Topography.  |  Pinto-Dueñas, DC., et al. 2024. Int J Mol Sci. 25: PMID: 38473701
9. Molecular alterations associated with pathophysiology in liver-specific ZO-1 and ZO-2 knockout mice.  |  Itoh, M., et al. 2024. Cell Struct Funct. 49: 83-99. PMID: 39322562
10. A ZO-2 scaffolding mechanism regulates the Hippo signalling pathway.  |  Liu, OX., et al. 2024. FEBS J.. PMID: 39462647

The preferred ZO-2 antibody is ZO-2 Antibody (E-3): sc-515115.