ZO-2 Antibody (E-3): sc-515115

ZO-2 Antibody (E-3) is a mouse monoclonal IgM antibody that detects ZO-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ZO-2 antibody (E-3) is available as the non-conjugated form. ZO-2 plays a crucial role in forming and maintaining tight junctions, which are essential for regulating paracellular permeability and maintaining epithelial and endothelial barrier integrity. Tight junctions comprise various proteins, including ZO-2, ZO-1, and claudins, that work together to create a selective barrier controlling ion and molecule movement between cells. Proper functioning of ZO-2 is vital for tissue homeostasis and cellular signaling, as disruptions in tight junction integrity can lead to various pathologies, including cancer metastasis and inflammatory diseases. ZO-2 is specifically localized at tight junctions of epithelial cells, where ZO-2 interacts with other tight junction proteins and actin cytoskeleton, contributing to structural stability and dynamic regulation of these junctions. ZO-2 and ZO-3 are expressed in epithelial tissues, while ZO-1 has a broader expression profile, including cardiac myocytes, highlighting specialized roles of these proteins in different cell types.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ZO-2 Antibody (E-3) References:

1. Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin.  |  Itoh, M., et al. 1999. J Biol Chem. 274: 5981-6. PMID: 10026224
2. zo-2 gene alternative promoters in normal and neoplastic human pancreatic duct cells.  |  Chlenski, A., et al. 1999. Int J Cancer. 83: 349-58. PMID: 10495427
3. Manner of interaction of heterogeneous claudin species within and between tight junction strands.  |  Furuse, M., et al. 1999. J Cell Biol. 147: 891-903. PMID: 10562289
4. Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3.  |  Wittchen, ES., et al. 1999. J Biol Chem. 274: 35179-85. PMID: 10575001
5. Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins.  |  Itoh, M., et al. 1999. J Cell Biol. 147: 1351-63. PMID: 10601346
6. Sorting nexin 27 (SNX27) associates with zonula occludens-2 (ZO-2) and modulates the epithelial tight junction.  |  Zimmerman, SP., et al. 2013. Biochem J. 455: 95-106. PMID: 23826934
7. ZO-1 and ZO-2 are required for extra-embryonic endoderm integrity, primitive ectoderm survival and normal cavitation in embryoid bodies derived from mouse embryonic stem cells.  |  Phua, DC., et al. 2014. PLoS One. 9: e99532. PMID: 24905925
8. Activation of the Ca2+ sensing receptor and the PKC/WNK4 downstream signaling cascade induces incorporation of ZO-2 to tight junctions and its separation from 14-3-3.  |  Amaya, E., et al. 2019. Mol Biol Cell. 30: 2377-2398. PMID: 31318316
9. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions.  |  Furuse, M., et al. 1994. J Cell Biol. 127: 1617-26. PMID: 7798316
10. Cell signalling: MAGUK magic.  |  Anderson, JM. 1996. Curr Biol. 6: 382-4. PMID: 8723338
11. ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin.  |  Haskins, J., et al. 1998. J Cell Biol. 141: 199-208. PMID: 9531559
12. Loss of the tight junction MAGUK ZO-1 in breast cancer: relationship to glandular differentiation and loss of heterozygosity.  |  Hoover, KB., et al. 1998. Am J Pathol. 153: 1767-73. PMID: 9846967

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZO-2 Antibody (E-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-ZO-2 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZO-2 Antibody (E-3) Specifications

Alternate Product Name: TJP2

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 789-813

Epitope Target: ZO-2

Epitope Species: rat

Product Reactivity/Detection: ZO-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for ZO-2 Antibody (E-3): P: Blocking Peptide