ZAP-70 Antibody (29): sc-136305
- ZAP-70 Antibody (29) is a mouse monoclonal IgG2a provided at 50 µg/0.5 ml
- raised against amino acids 468-619 of ZAP-70 of human origin
- recommended for detection of ZAP-70 of mouse, rat and human origin by WBIP
- See ZAP-70 (1E7.2): sc-32760 for ZAP-70 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ZAP-70 Antibody (29). This work is in progress.
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ZAP-70 Antibody (29) is a mouse monoclonal IgG2a light chain antibody that detects ZAP-70 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. ZAP-70, a crucial tyrosine kinase, plays a significant role in T cell receptor signaling, which is essential for T lymphocyte activation and subsequent immune responses. Located primarily in the cytoplasm, ZAP-70 associates with the ζ chain of the T cell receptor complex, where ZAP-70 becomes activated following TCR stimulation. This activation is vital as ZAP-70 phosphorylates various downstream signaling molecules, thereby propagating the signal necessary for T cell activation and proliferation. The proper functioning of ZAP-70 is critical for effective immune responses, and dysregulation can lead to immunodeficiencies or autoimmune diseases. Anti-ZAP-70 antibody (29) is an invaluable tool for researchers studying T cell signaling pathways and their implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZAP-70 Antibody (29) References:
- Immunohistochemical detection of ZAP-70 in 341 cases of non-Hodgkin and Hodgkin lymphoma. | Admirand, JH., et al. 2004. Mod Pathol. 17: 954-61. PMID: 15133473
- Functional and prognostic role of ZAP-70 in chronic lymphocytic leukaemia. | Hamblin, AD. and Hamblin, TJ. 2005. Expert Opin Ther Targets. 9: 1165-78. PMID: 16300468
- Clinical implications of ZAP-70 expressionin chronic lymphocytic leukemia. | Bosch, F., et al. 2006. Cytometry B Clin Cytom. 70: 214-7. PMID: 16906580
- ZAP-70 tyrosines 315 and 492 transmit non-genomic glucocorticoid (GC) effects in T cells. | Boldizsar, F., et al. 2013. Mol Immunol. 53: 111-7. PMID: 22898186
- The SKG Mutation in ZAP-70 also Confers Arthritis Susceptibility in C57 Black Mouse Strains. | Guerard, S., et al. 2016. Scand J Immunol. 84: 3-11. PMID: 27040161
- Imaging Spatiotemporal Activities of ZAP-70 in Live T Cells Using a FRET-Based Biosensor. | Li, K., et al. 2016. Ann Biomed Eng. 44: 3510-3521. PMID: 27384937
- Identification of Inhibitors of the Association of ZAP-70 with the T Cell Receptor by High-Throughput Screen. | Visperas, PR., et al. 2017. SLAS Discov. 22: 324-331. PMID: 27932698
- Flow Cytometric Analysis of ZAP-70 Protein Expression for B-Cell Chronic Lymphocytic Leukemia Prognostication: Usefulness and Limitations. | Shaikh, MS., et al. 2020. Cureus. 12: e11691. PMID: 33391925
- Lymphocyte disturbance and functional assessment of the [Asp521Asn] ZAP70 mutation. | Lin, YF., et al. 2023. Clin Immunol. 247: 109236. PMID: 36669607
- ZAP-70 and defects of T-cell receptor signaling. | Elder, ME. 1998. Semin Hematol. 35: 310-20. PMID: 9801260
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZAP-70 Antibody (29) | sc-136305 | 50 µg/0.5 ml | $316.00 |