Date published: 2026-4-24

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ZAG Antibody (F-6): sc-271957

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Datasheets
  • ZAG Antibody (F-6) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 257-279 near the C-terminus of ZAG of mouse origin
  • ZAG Antibody (F-6) is recommended for detection of ZAG of mouse origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-ZAG Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for ZAG Antibody (F-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with ZAG Antibody (F-6) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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    ZAG Antibody (F-6) is a mouse monoclonal IgG1 antibody that detects ZAG in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ZAG (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZAG, or Zn-a2-glycoprotein, is a soluble protein predominantly found in serum and other bodily fluids, including cerebrospinal fluid, blood plasma, urine, and sweat. ZAG plays a crucial role in lipid metabolism, particularly in adipocytes, where ZAG stimulates lipid degradation. ZAG overexpression is linked to significant fat loss in patients with advanced cancers, highlighting ZAG′s potential as a biomarker for cachexia and metabolic disorders. ZAG is a member of the immunoglobulin superfamily and shares a high degree of sequence similarity with class-I major histocompatibility complex (MHC) antigens, suggesting ZAG′s involvement in immune responses. ZAG′s unique structural features, including a large groove similar to the peptide binding grooves of class I MHC, further underscore ZAG′s functional significance in cellular processes.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    ZAG Antibody (F-6)

    sc-271957
    200 µg/ml
    $322.00

    ZAG Antibody (F-6): m-IgGλ BP-HRP Bundle

    sc-551134
    200 µg Ab; 40 µg BP
    $361.00

    ZAG Antibody (F-6) AC

    sc-271957 AC
    500 µg/ml, 25% agarose
    $424.00

    ZAG Antibody (F-6) HRP

    sc-271957 HRP
    200 µg/ml
    $322.00

    ZAG Antibody (F-6) FITC

    sc-271957 FITC
    200 µg/ml
    $336.00

    ZAG Antibody (F-6) PE

    sc-271957 PE
    200 µg/ml
    $349.00

    ZAG Antibody (F-6) Alexa Fluor® 488

    sc-271957 AF488
    200 µg/ml
    $364.00

    ZAG Antibody (F-6) Alexa Fluor® 546

    sc-271957 AF546
    200 µg/ml
    $364.00

    ZAG Antibody (F-6) Alexa Fluor® 594

    sc-271957 AF594
    200 µg/ml
    $364.00

    ZAG Antibody (F-6) Alexa Fluor® 647

    sc-271957 AF647
    200 µg/ml
    $364.00

    ZAG Antibody (F-6) Alexa Fluor® 680

    sc-271957 AF680
    200 µg/ml
    $364.00

    ZAG Antibody (F-6) Alexa Fluor® 790

    sc-271957 AF790
    200 µg/ml
    $364.00

    ZAG (F-6) Neutralizing Peptide

    sc-271957 P
    100 µg/0.5 ml
    $69.00

    What application is the blocking peptide sc-271957 P appropriate for?

    Asked by: Germaine
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
    • y_2026, m_4, d_23, h_7CST
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    Rated 1 out of 5 by from DisappointedI got it as a trial sample since they discontinued the polyclonal ZAG antibody (E-20), which worked perfectly fine, I did not get any band in the over expressing ZAG cell seven after using 1:100 dilution.
    Date published: 2017-08-15
    Rated 5 out of 5 by from Produced nice Western blot data of ZAG expressionProduced nice Western blot data of ZAG expression in human PBL whole cell lysate. -SCBT QC
    Date published: 2014-11-23
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    ZAG Antibody (F-6) is rated 3.0 out of 5 by 2.
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