Anti-ZAG Antibody (1D4) recommended for detection of ZAG of human origin by WB, IP, IF and IHC(P)

Anti-ZAG Antibody (1D4): sc-13585

Anti-ZAG Antibody (1D4) is rated 4.3 out of 5 by 3.
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Datasheets

    • Anti-ZAG Antibody (1D4) is a mouse monoclonal IgG1 κ ZAG antibody, cited in 12 publications, provided at 200 µg/ml
    • raised against full length ZAG of human origin
    • ZAG Antibody (1D4) is recommended for detection of ZAG of human origin by WB, IP, IF and IHC(P)
    • Anti-ZAG Antibody (1D4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ZAG (1D4): sc-13585.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ZAG Antibody (1D4) for WB and IHC(P) applications. These reagents are now offered in bundles with ZAG Antibody (1D4) (see ordering information below).
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I am using ZAG (1D4): sc-13585 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Consistent band in western blot We used this antibody for western blot and got continually got a nice band for the correct molecular weight
Date published: 2017-01-16
Rated 4 out of 5 by from Clear immunoperoxidase cytoplasmic staining Clear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Date published: 2015-04-30
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