XRN1 Antibody (C-1): sc-165985

XRN1 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects XRN1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). XRN1 Antibody (C-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XRN1, also known as Exoribonuclease I, Sep1, or Rar5, is a crucial 1,694-amino acid enzyme that primarily functions as the major cytoplasmic 5′ to 3′ exoribonuclease, playing a vital role in mRNA turnover and degradation, which is essential for regulating gene expression. XRN1 not only facilitates the decay of mRNA but also contributes to the formation of stress granules and processing bodies, which are critical for cellular responses to stress and mRNA regulation. XRN1 is involved in the microtubular cytoskeleton and has implications in DNA recombination and replication processes. Loss of XRN1 can lead to detrimental effects on cell growth rates, underscoring XRN1′s importance in maintaining cellular homeostasis and function.

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XRN1 Antibody (C-1) References:

1. Inhibition of mRNA turnover in yeast by an xrn1 mutation enhances the requirement for eIF4E binding to eIF4G and for proper capping of transcripts by Ceg1p.  |  Brown, JT., et al. 2000. Genetics. 155: 31-42. PMID: 10790382
2. 5'-exoribonuclease 1: Xrn1.  |  Stevens, A. 2001. Methods Enzymol. 342: 251-9. PMID: 11586897
3. mRNA decay: x (XRN1) marks the spot.  |  Long, RM. and McNally, MT. 2003. Mol Cell. 11: 1126-8. PMID: 12769838
4. Decay of mRNAs targeted by RISC requires XRN1, the Ski complex, and the exosome.  |  Orban, TI. and Izaurralde, E. 2005. RNA. 11: 459-69. PMID: 15703439
5. Stress granules and processing bodies are dynamically linked sites of mRNP remodeling.  |  Kedersha, N., et al. 2005. J Cell Biol. 169: 871-84. PMID: 15967811
6. Regulation and intracellular localization of Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multifunctional exonuclease.  |  Heyer, WD., et al. 1995. Mol Cell Biol. 15: 2728-36. PMID: 7739553
7. Identification of functional domains in the Sep1 protein (= Kem1, Xrn1), which is required for transition through meiotic prophase in Saccharomyces cerevisiae.  |  Bashkirov, VI., et al. 1995. Chromosoma. 104: 215-22. PMID: 8529461
8. Structural modifications of RNA influence the 5' exoribonucleolytic hydrolysis by XRN1 and HKE1 of Saccharomyces cerevisiae.  |  Poole, TL. and Stevens, A. 1997. Biochem Biophys Res Commun. 235: 799-805. PMID: 9207242

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XRN1 Antibody (C-1) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-XRN1 Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XRN1 Antibody (C-1) Specifications

Alternate Product Name: XRN1

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 301-450

Epitope Target: XRN1

Epitope Species: human

Product Reactivity/Detection: XRN1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for XRN1 Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin