XPG Antibody (8H7): sc-13563

XPG Antibody (8H7) is a mouse monoclonal IgG2a kappa light chain antibody that detects XPG protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). XPG (8H7) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. XPG protein, also known as ERCC5, plays a crucial role in the nucleotide excision repair pathway, specifically making the 3′ incision in damaged DNA. This function is vital for maintaining genomic stability, as XPG helps to repair DNA lesions caused by environmental factors such as ultraviolet radiation and chemical agents. Mutations in XPG gene can lead to xeroderma pigmentosum, a condition characterized by extreme sensitivity to sunlight and a predisposition to skin cancers. Structural integrity of XPG is essential for endonuclease activity, as conserved residues in catalytic center are critical for function in DNA repair. XPG (8H7) antibody enables researchers to effectively study expression and localization of XPG in various biological contexts, contributing to a deeper understanding of DNA repair mechanisms and implications in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

XPG Antibody (8H7) References:

1. Conserved residues of human XPG protein important for nuclease activity and function in nucleotide excision repair.  |  Constantinou, A., et al. 1999. J Biol Chem. 274: 5637-48. PMID: 10026181
2. The relative expression of mutated XPB genes results in xeroderma pigmentosum/Cockayne's syndrome or trichothiodystrophy cellular phenotypes.  |  Riou, L., et al. 1999. Hum Mol Genet. 8: 1125-33. PMID: 10332046
3. The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta.  |  Masutani, C., et al. 1999. Nature. 399: 700-4. PMID: 10385124
4. XPG genetic polymorphisms and clinical outcome of patients with advanced non-small cell lung cancer under platinum-based treatment: a meta-analysis of 12 studies.  |  Xiang, T., et al. 2017. Cancer Chemother Pharmacol. 79: 791-800. PMID: 28314991
5. Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.  |  Yasuhara, T., et al. 2018. Cell. 175: 558-570.e11. PMID: 30245011
6. An ERCC5 gene with homology to yeast RAD2 is involved in group G xeroderma pigmentosum.  |  Shiomi, T., et al. 1994. Mutat Res. 314: 167-75. PMID: 7510366
7. High incidence of ultraviolet-B-or chemical-carcinogen-induced skin tumours in mice lacking the xeroderma pigmentosum group A gene.  |  Nakane, H., et al. 1995. Nature. 377: 165-8. PMID: 7675085
8. Mutations in XPA that prevent association with ERCC1 are defective in nucleotide excision repair.  |  Li, L., et al. 1995. Mol Cell Biol. 15: 1993-8. PMID: 7891694
9. Complementation of the DNA repair defect in xeroderma pigmentosum group G cells by a human cDNA related to yeast RAD2.  |  Scherly, D., et al. 1993. Nature. 363: 182-5. PMID: 8483504
10. Identification of a damaged-DNA binding domain of the XPA protein.  |  Kuraoka, I., et al. 1996. Mutat Res. 362: 87-95. PMID: 8538652
11. Separation of protein factors that correct the defects in the seven complementation groups of xeroderma pigmentosum cells.  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098
12. The DNA helicases acting in nucleotide excision repair, XPD, CSB and XPB, are not required for PCNA-dependent repair of abasic sites.  |  Cappelli, E., et al. 1999. Eur J Biochem. 259: 325-30. PMID: 9914510

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XPG Antibody (8H7) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-XPG Antibody (8H7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XPG Antibody (8H7) Specifications

Alternate Product Name: XPG

Product Clone Name: 8H7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: XPG

Epitope Species: human

Product Reactivity/Detection: XPG

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for XPG Antibody (8H7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin