XPF Antibody (F-11): sc-398032
- XPF Antibody (F-11) is a mouse monoclonal IgG2a κ XPF antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 888-911 at the C-terminus of XPF of mouse origin
- XPF Antibody (F-11) is recommended for detection of XPF of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-XPF Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for XPF Antibody (F-11) for WB applications. These reagents are now offered in bundles with XPF Antibody (F-11) (see ordering information below).
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XPF Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects XPF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). XPF (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XPF protein, also known as ERCC4 or ERCC11, plays a crucial role in the nucleotide excision repair pathway, which is essential for maintaining genomic stability by repairing DNA damage caused by ultraviolet light and other environmental factors. Specifically, XPF forms a complex with ERCC1 protein, functioning as a structure-specific endonuclease that makes incisions in DNA during the repair process. This interaction allows for precise removal of damaged DNA segments, thereby preventing mutations that could lead to conditions such as xeroderma pigmentosum, an autosomal recessive disorder characterized by heightened risk of skin cancer due to impaired DNA repair mechanisms. XPF′s ability to cleave DNA at specific sites underscores its importance in cellular response to DNA damage, making anti-XPF antibody (F-11) an invaluable tool for researchers studying DNA repair processes and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
XPF Antibody (F-11) References:
- Multiple roles of the ERCC1-XPF endonuclease in DNA repair and resistance to anticancer drugs. | Kirschner, K. and Melton, DW. 2010. Anticancer Res. 30: 3223-32. PMID: 20944091
- Physiological consequences of defects in ERCC1-XPF DNA repair endonuclease. | Gregg, SQ., et al. 2011. DNA Repair (Amst). 10: 781-91. PMID: 21612988
- Association between XPF polymorphisms and cancer risk: a meta-analysis. | Shi, TY., et al. 2012. PLoS One. 7: e38606. PMID: 22768293
- DNA repair endonuclease ERCC1-XPF as a novel therapeutic target to overcome chemoresistance in cancer therapy. | McNeil, EM. and Melton, DW. 2012. Nucleic Acids Res. 40: 9990-10004. PMID: 22941649
- Functional Comparison of XPF Missense Mutations Associated to Multiple DNA Repair Disorders. | Marín, M., et al. 2019. Genes (Basel). 10: PMID: 30658521
- XPF expression and its relationship with the risk and prognosis of colorectal cancer. | Hu, H., et al. 2021. Cancer Cell Int. 21: 12. PMID: 33407486
- Tissue-Specific DNA Repair Activity of ERCC-1/XPF-1. | Sabatella, M., et al. 2021. Cell Rep. 34: 108608. PMID: 33440146
- The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing. | Goulielmaki, E., et al. 2021. Nat Commun. 12: 3153. PMID: 34039990
- The structure-specific endonuclease complex SLX4-XPF regulates Tus-Ter-induced homologous recombination. | Elango, R., et al. 2022. Nat Struct Mol Biol. 29: 801-812. PMID: 35941380
- XPF-ERCC1 Blocker Improves the Therapeutic Efficacy of 5-FU- and Oxaliplatin-Based Chemoradiotherapy in Colorectal Cancer. | Huang, MY., et al. 2023. Cells. 12: PMID: 37296596
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPF Antibody (F-11) | sc-398032 | 200 µg/ml | $316.00 | |||
XPF Antibody (F-11): m-IgG Fc BP-HRP Bundle | sc-530672 | 200 µg Ab; 10 µg BP | $354.00 | |||
XPF Antibody (F-11): m-IgGκ BP-HRP Bundle | sc-524192 | 200 µg Ab, 40 µg BP | $354.00 | |||
XPF Antibody (F-11): m-IgG2a BP-HRP Bundle | sc-547554 | 200 µg Ab; 10 µg BP | $354.00 | |||
XPF Antibody (F-11) AC | sc-398032 AC | 500 µg/ml, 25% agarose | $416.00 | |||
XPF Antibody (F-11) HRP | sc-398032 HRP | 200 µg/ml | $316.00 | |||
XPF Antibody (F-11) FITC | sc-398032 FITC | 200 µg/ml | $330.00 | |||
XPF Antibody (F-11) PE | sc-398032 PE | 200 µg/ml | $343.00 | |||
XPF Antibody (F-11) Alexa Fluor® 488 | sc-398032 AF488 | 200 µg/ml | $357.00 | |||
XPF Antibody (F-11) Alexa Fluor® 546 | sc-398032 AF546 | 200 µg/ml | $357.00 | |||
XPF Antibody (F-11) Alexa Fluor® 594 | sc-398032 AF594 | 200 µg/ml | $357.00 | |||
XPF Antibody (F-11) Alexa Fluor® 647 | sc-398032 AF647 | 200 µg/ml | $357.00 | |||
XPF Antibody (F-11) Alexa Fluor® 680 | sc-398032 AF680 | 200 µg/ml | $357.00 | |||
XPF Antibody (F-11) Alexa Fluor® 790 | sc-398032 AF790 | 200 µg/ml | $357.00 | |||
XPF (F-11) Neutralizing Peptide | sc-398032 P | 100 µg/0.5 ml | $68.00 |