XPF Antibody (3F2/3): sc-136153

XPF Antibody (3F2/3) is a mouse monoclonal IgG1 kappa light chain antibody that detects XPF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). XPF (3F2/3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XPF, also known as ERCC4 or ERCC11, plays a crucial role in the nucleotide excision repair pathway, which is essential for maintaining genomic stability by repairing DNA damage caused by ultraviolet light and other environmental factors. Specifically, XPF forms a complex with the ERCC1 protein, which is vital for the endonuclease activity that incises damaged DNA. This interaction is critical because XPF-ERCC1 complex recognizes and cleaves DNA at specific sites, facilitating the removal of bulky DNA adducts and ensuring proper repair. Deficiencies in XPF are linked to xeroderma pigmentosum, an autosomal recessive disorder that significantly increases the risk of skin cancer due to impaired DNA repair mechanisms. Anti-XPF antibody (3F2/3) enables researchers to gain insights into the molecular mechanisms underlying DNA repair and the implications of XPF dysfunction in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

XPF Antibody (3F2/3) References:

1. Action of DNA repair endonuclease ERCC1/XPF in living cells.  |  Houtsmuller, AB., et al. 1999. Science. 284: 958-61. PMID: 10320375
2. Order of assembly of human DNA repair excision nuclease.  |  Wakasugi, M. and Sancar, A. 1999. J Biol Chem. 274: 18759-68. PMID: 10373492
3. Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10.  |  van Duin, M., et al. 1986. Cell. 44: 913-23. PMID: 2420469
4. Function and Interactions of ERCC1-XPF in DNA Damage Response.  |  Faridounnia, M., et al. 2018. Molecules. 23: PMID: 30563071
5. Impact of XPF rs2276466 polymorphism on cancer susceptibility: a meta-analysis.  |  Liu, Y., et al. 2019. Biosci Rep. 39: PMID: 31040199
6. XPF -673C>T variation is associated with the susceptibility to breast cancer.  |  Wu, H., et al. 2021. Cancer Epidemiol. 74: 102007. PMID: 34416547
7. XPF activates break-induced telomere synthesis.  |  Guh, CY., et al. 2022. Nat Commun. 13: 5781. PMID: 36184605
8. XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes.  |  Chatzinikolaou, G., et al. 2023. Sci Adv. 9: eadi2095. PMID: 37939182
9. Mammalian DNA nucleotide excision repair reconstituted with purified protein components.  |  Aboussekhra, A., et al. 1995. Cell. 80: 859-68. PMID: 7697716
10. Mutations in XPA that prevent association with ERCC1 are defective in nucleotide excision repair.  |  Li, L., et al. 1995. Mol Cell Biol. 15: 1993-8. PMID: 7891694
11. Separation of protein factors that correct the defects in the seven complementation groups of xeroderma pigmentosum cells.  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098
12. Xeroderma pigmentosum group F caused by a defect in a structure-specific DNA repair endonuclease.  |  Sijbers, AM., et al. 1996. Cell. 86: 811-22. PMID: 8797827

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XPF Antibody (3F2/3) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-XPF Antibody (3F2/3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XPF Antibody (3F2/3) Specifications

Alternate Product Name: XPF

Product Clone Name: 3F2/3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 629-905

Epitope Target: XPF

Epitope Species: human

Product Reactivity/Detection: XPF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for XPF Antibody (3F2/3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred XPF antibody is XPF Antibody (F-11): sc-398032.