WAP Antibody (E-8): sc-398276

WAP Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Whey Acidic Protein (WAP) of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WAP Antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WAP is a hormonally-regulated, acidic, cysteine-rich protein primarily found in rodent milk, and plays a crucial role in protease activity regulation, essential for maintaining protein homeostasis during lactation. The WAP gene, consisting of four exons and three introns, encodes two distinct cysteine-rich domains that contribute to structural integrity and functional properties. WAP is expressed in mammary epithelial cells and is significantly induced during pregnancy under the influence of lactogenic hormones such as glucocorticoids, prolactin, and insulin. WAP mRNA expression is tightly regulated and varies with the estrous cycle, being predominantly detected during the midcycle phase. While low levels of WAP RNA can be found in some non-mammary tissues, such as the tongue, pancreas, and pituitary gland, WAP is absent in others like the heart and brain. WAP secretion into milk occurs throughout lactation and is specific to certain species, including mouse, rat, rabbit, camel, and porcine. Mouse mammary epithelial cells cultured on a basement membrane-type matrix express high levels of WAP mRNA and secrete WAP into the lumen, a process dependent on alveoli-like structure formation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WAP Antibody (E-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-WAP Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WAP Antibody (E-8) Specifications

Alternate Product Name: WAP

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 60-89

Epitope Target: WAP

Epitope Species: mouse

Product Reactivity/Detection: WAP

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for WAP Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred WAP antibody is WAP Antibody (C-10): sc-374648.