WAP Antibody (C-10): sc-374648

WAP Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects WAP protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WAP (C-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Whey acidic protein (WAP) is a hormonally-regulated, acidic, cysteine-rich protein primarily found in rodent milk, and WAP plays a crucial role in regulating protease activity, essential for maintaining milk protein integrity during lactation. WAP gene, consisting of four exons and three introns, encodes two distinct cysteine-rich domains that contribute to WAP′s function as a protease inhibitor. WAP contains a 19-amino acid N-terminal signal peptide that directs secretion. WAP gene expression is significantly induced during pregnancy, driven by lactogenic hormones such as glucocorticoids, prolactin, and insulin, and remains tightly regulated throughout the estrous cycle, with mRNA levels peaking during midcycle. While WAP expression occurs predominantly in mammary epithelial cells, low levels of WAP RNA can be detected in some non-mammary tissues, including the tongue, pancreas, and pituitary gland, but not in the heart or brain. WAP secretion into milk occurs throughout lactation and is specific to several species, including mouse, rat, rabbit, camel, and porcine. Mouse mammary epithelial cells cultured on a basement membrane-type matrix express high levels of WAP mRNA and secrete WAP into the lumen, a process dependent on alveoli-like structure formation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

WAP Antibody (C-10) References:

1. The gene for a novel member of the whey acidic protein family encodes three four-disulfide core domains and is asynchronously expressed during lactation.  |  Simpson, KJ., et al. 2000. J Biol Chem. 275: 23074-81. PMID: 10801834
2. Estrous cycle regulation of mammary epithelial cell proliferation, differentiation, and death in the Sprague-Dawley rat: a model for investigating the role of estrous cycling in mammary carcinogenesis.  |  Schedin, P., et al. 2000. J Mammary Gland Biol Neoplasia. 5: 211-25. PMID: 11149574
3. Lactogenic hormone and cell type-specific control of the whey acidic protein gene promoter in transfected mouse cells.  |  Doppler, W., et al. 1991. Mol Endocrinol. 5: 1624-32. PMID: 1685765
4. Regulation of expression of genes for milk proteins.  |  Hennighausen, L., et al. 1991. Biotechnology. 16: 65-74. PMID: 2007204
5. Ha-ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation, and tumor induction in transgenic mice.  |  Andres, AC., et al. 1987. Proc Natl Acad Sci U S A. 84: 1299-303. PMID: 3469670
6. Comparison of the whey acidic protein genes of the rat and mouse.  |  Campbell, SM., et al. 1984. Nucleic Acids Res. 12: 8685-97. PMID: 6095207
7. Mouse whey acidic protein is a novel member of the family of 'four-disulfide core' proteins.  |  Hennighausen, LG. and Sippel, AE. 1982. Nucleic Acids Res. 10: 2677-84. PMID: 6896234
8. Comparative sequence analysis of the mRNAs coding for mouse and rat whey protein.  |  Hennighausen, LG., et al. 1982. Nucleic Acids Res. 10: 3733-44. PMID: 6896749
9. A novel regulatory mechanism for whey acidic protein gene expression.  |  Chen, LH. and Bissell, MJ. 1989. Cell Regul. 1: 45-54. PMID: 2519617

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WAP Antibody (C-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-WAP Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WAP Antibody (C-10) Specifications

Alternate Product Name: WAP

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 7-137

Epitope Target: WAP

Epitope Species: rat

Product Reactivity/Detection: WAP

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for WAP Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin