VRK1 Antibody (A-11): sc-271061
- VRK1 Antibody (A-11) is a mouse monoclonal IgG1 κ VRK1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against a peptide mapping within an internal region of VRK1 of human origin
- VRK1 Antibody (A-11) is recommended for detection of VRK1 of human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- Anti-VRK1 Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VRK1 Antibody (A-11) for WB and IHC(P) applications. These reagents are now offered in bundles with VRK1 Antibody (A-11) (see ordering information below).
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VRK1 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects VRK1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). VRK1 (A-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VRK1 plays a crucial role in cellular stress responses by phosphorylating key transcription factors, including p53 and ATF2, which are vital for regulating cell cycle and apoptosis. Specifically, VRK1 phosphorylates p53 at Thr18, disrupting p53′s interaction with Mdm2, thereby stabilizing p53 and enhancing tumor suppressor functions. Additionally, VRK1′s interaction with ATF2, where VRK1 phosphorylates Thr73, not only stabilizes ATF2 but also promotes transcriptional activity, which is essential for cellular responses to stress. VRK1′s ability to modulate these critical pathways underscores its importance in cancer biology and cellular homeostasis, making anti-VRK1 antibody (A-11) an invaluable tool for researchers studying these processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VRK1 Antibody (A-11) References:
- The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein. | Lopez-Borges, S. and Lazo, PA. 2000. Oncogene. 19: 3656-64. PMID: 10951572
- Characterization of three paralogous members of the Mammalian vaccinia related kinase family. | Nichols, RJ. and Traktman, P. 2004. J Biol Chem. 279: 7934-46. PMID: 14645249
- Members of a novel family of mammalian protein kinases complement the DNA-negative phenotype of a vaccinia virus ts mutant defective in the B1 kinase. | Boyle, KA. and Traktman, P. 2004. J Virol. 78: 1992-2005. PMID: 14747564
- Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK. | Sevilla, A., et al. 2004. J Biol Chem. 279: 27458-65. PMID: 15105425
- c-Jun phosphorylation by the human vaccinia-related kinase 1 (VRK1) and its cooperation with the N-terminal kinase of c-Jun (JNK). | Sevilla, A., et al. 2004. Oncogene. 23: 8950-8. PMID: 15378002
- p53 Stabilization and accumulation induced by human vaccinia-related kinase 1. | Vega, FM., et al. 2004. Mol Cell Biol. 24: 10366-80. PMID: 15542844
- Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. | Hunter, T. 1995. Cell. 80: 225-36. PMID: 7834742
- Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase. | Nezu, J., et al. 1997. Genomics. 45: 327-31. PMID: 9344656
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VRK1 Antibody (A-11) | sc-271061 | 200 µg/ml | $322.00 | |||
VRK1 Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-529110 | 200 µg Ab; 10 µg BP | $361.00 | |||
VRK1 Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-521835 | 200 µg Ab, 40 µg BP | $361.00 | |||
VRK1 Antibody (A-11): m-IgG1 BP-HRP Bundle | sc-543325 | 200 µg Ab; 20 µg BP | $361.00 | |||
VRK1 Antibody (A-11) AC | sc-271061 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VRK1 Antibody (A-11) HRP | sc-271061 HRP | 200 µg/ml | $322.00 | |||
VRK1 Antibody (A-11) FITC | sc-271061 FITC | 200 µg/ml | $336.00 | |||
VRK1 Antibody (A-11) PE | sc-271061 PE | 200 µg/ml | $349.00 | |||
VRK1 Antibody (A-11) Alexa Fluor® 488 | sc-271061 AF488 | 200 µg/ml | $364.00 | |||
VRK1 Antibody (A-11) Alexa Fluor® 546 | sc-271061 AF546 | 200 µg/ml | $364.00 | |||
VRK1 Antibody (A-11) Alexa Fluor® 594 | sc-271061 AF594 | 200 µg/ml | $364.00 | |||
VRK1 Antibody (A-11) Alexa Fluor® 647 | sc-271061 AF647 | 200 µg/ml | $364.00 | |||
VRK1 Antibody (A-11) Alexa Fluor® 680 | sc-271061 AF680 | 200 µg/ml | $364.00 | |||
VRK1 Antibody (A-11) Alexa Fluor® 790 | sc-271061 AF790 | 200 µg/ml | $364.00 | |||
VRK1 (A-11) Neutralizing Peptide | sc-271061 P | 100 µg/0.5 ml | $69.00 |