VPS37B Antibody (D-4): sc-390144
- VPS37B Antibody (D-4) is a mouse monoclonal IgG2b κ VPS37B antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 235-268 within an internal region of VPS37B of human origin
- VPS37B Antibody (D-4) is recommended for detection of VPS37B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-VPS37B Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VPS37B Antibody (D-4) for WB applications. These reagents are now offered in bundles with VPS37B Antibody (D-4) (see ordering information below).
VPS37B Antibody (D-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects VPS37B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VPS37B Antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VPS37B, or vacuolar protein sorting 37 homolog B, is a crucial component of the endosomal sorting complex required for transport (ESCRT-I), which plays a significant role in sorting ubiquitinated proteins into multivesicular bodies. This process is vital for maintaining cellular homeostasis and regulating membrane protein degradation, thereby influencing cell growth and differentiation. VPS37B protein, consisting of 285 amino acids, is primarily expressed in macrophages and lymphocytes, where VPS37B is recruited to the endosomal membrane in a VPS4A-dependent manner. Proper functioning of VPS37B is essential for trafficking endocytic and biosynthetic proteins to the vacuole, highlighting VPS37B′s importance in cellular processes such as signal transduction and nutrient uptake.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VPS37B Antibody (D-4) References:
- The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding. | Stuchell, MD., et al. 2004. J Biol Chem. 279: 36059-71. PMID: 15218037
- The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation. | Bache, KG., et al. 2004. Mol Biol Cell. 15: 4337-46. PMID: 15240819
- Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding. | Eastman, SW., et al. 2005. J Biol Chem. 280: 628-36. PMID: 15509564
- Structural studies of phosphoinositide 3-kinase-dependent traffic to multivesicular bodies. | Gill, DJ., et al. 2007. Biochem Soc Symp. 47-57. PMID: 17233579
- Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. | Morita, E., et al. 2007. EMBO J. 26: 4215-27. PMID: 17853893
- Involvement of vacuolar protein sorting pathway in Ebola virus release independent of TSG101 interaction. | Silvestri, LS., et al. 2007. J Infect Dis. 196 Suppl 2: S264-70. PMID: 17940959
- Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. | Morita, E., et al. 2007. Cell Host Microbe. 2: 41-53. PMID: 18005716
- NEDD4L overexpression rescues the release and infectivity of human immunodeficiency virus type 1 constructs lacking PTAP and YPXL late domains. | Chung, HY., et al. 2008. J Virol. 82: 4884-97. PMID: 18321968
- Biochemical analyses of human IST1 and its function in cytokinesis. | Bajorek, M., et al. 2009. Mol Biol Cell. 20: 1360-73. PMID: 19129479
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS37B Antibody (D-4) | sc-390144 | 200 µg/ml | $322.00 | |||
VPS37B Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-523424 | 200 µg Ab, 40 µg BP | $361.00 | |||
VPS37B Antibody (D-4): m-IgG2b BP-HRP Bundle | sc-549117 | 200 µg Ab; 10 µg BP | $361.00 | |||
VPS37B Antibody (D-4) AC | sc-390144 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VPS37B Antibody (D-4) HRP | sc-390144 HRP | 200 µg/ml | $322.00 | |||
VPS37B Antibody (D-4) FITC | sc-390144 FITC | 200 µg/ml | $336.00 | |||
VPS37B Antibody (D-4) PE | sc-390144 PE | 200 µg/ml | $349.00 | |||
VPS37B Antibody (D-4) Alexa Fluor® 488 | sc-390144 AF488 | 200 µg/ml | $364.00 | |||
VPS37B Antibody (D-4) Alexa Fluor® 546 | sc-390144 AF546 | 200 µg/ml | $364.00 | |||
VPS37B Antibody (D-4) Alexa Fluor® 594 | sc-390144 AF594 | 200 µg/ml | $364.00 | |||
VPS37B Antibody (D-4) Alexa Fluor® 647 | sc-390144 AF647 | 200 µg/ml | $364.00 | |||
VPS37B Antibody (D-4) Alexa Fluor® 680 | sc-390144 AF680 | 200 µg/ml | $364.00 | |||
VPS37B Antibody (D-4) Alexa Fluor® 790 | sc-390144 AF790 | 200 µg/ml | $364.00 | |||
VPS37B (D-4) Neutralizing Peptide | sc-390144 P | 100 µg/0.5 ml | $69.00 |