Vimentin Antibody (3CB2) recommended for detection of Vimentin of chicken, human, mouse and rat origin by WB, IP, IF and IHC(P)

Vimentin Antibody (3CB2): sc-80975

Vimentin Antibody (3CB2) is rated 4.0 out of 5 by 4.
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Datasheets

    • Vimentin Antibody (3CB2) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against Vimentin extracted from embryonic brain tissue of chicken origin
    • recommended for detection of Vimentin of chicken, human, mouse and rat origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Vimentin (E-5): sc-373717 for Vimentin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using Vimentin (3CB2): sc-80975 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from A crisp band was observed by Western Blot A crisp band was observed by Western Blot of the endogenously expressed vinculin protein in HUV-EC-C whole cell lysates. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Produced great immunofluorescence cytoskeletal Produced great immunofluorescence cytoskeletal staining in paraformaldehyde-fixed cultured human U-87 glioma cells. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Multiple bands (protein is glycosylated and Multiple bands (protein is glycosylated and phosphorylated post translation) observed by Western Blot using detergent insoluble extracts from human U-87 glioma cells. -SCBT QC
Date published: 2015-02-03
Rated 4 out of 5 by from Specificity demonstrated by Western Blot Specificity demonstrated by Western Blot comparing 293T cells that have been transfected to over-express the human vinculin protein to those that have not. A definite increase in expression post transfection is observed. -SCBT QC
Date published: 2015-02-02
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