Vimentin Activators
Vimentin Activators refers to a group of chemical compounds and agents that have been found to modulate the activity and phosphorylation status of Vimentin, a key intermediate filament protein in the cytoskeleton of eukaryotic cells. Vimentin is known for its crucial role in maintaining cellular structure and integrity, particularly in mesenchymal cells. These activators are not a specific chemical class themselves but encompass a diverse range of molecules with distinct mechanisms of action. They play a significant role in cell biology research by offering insights into the dynamic regulation of Vimentin and its impact on cellular functions. One common mechanism among Vimentin activators is the induction of post-translational modifications, primarily phosphorylation, which influences Vimentin's polymerization and reorganization. Compounds like Phorbol 12-myristate 13-acetate (PMA) and Okadaic Acid stimulate protein kinases, such as protein kinase C (PKC), leading to the phosphorylation of Vimentin. This phosphorylation can trigger the remodeling of the cytoskeleton, affecting cell motility and shape.
Additionally, agents like Calyculin A act as phosphatase inhibitors, preventing the dephosphorylation of Vimentin and maintaining it in a phosphorylated state. Furthermore, some Vimentin activators, like Forskolin, elevate intracellular cyclic AMP (cAMP) levels, which can activate protein kinases that target Vimentin. Others, such as A23187 (Calcimycin), increase intracellular calcium concentrations, subsequently activating calcium-dependent kinases involved in Vimentin phosphorylation. The diversity of these activators provides researchers with valuable tools to dissect the intricate regulatory mechanisms governing Vimentin and its involvement in various cellular processes, including cell migration, intracellular transport, and response to external stimuli. Understanding how these compounds impact Vimentin dynamics contributes to the broader comprehension of cytoskeletal dynamics and cell biology as a whole.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Activates Vimentin by stimulating protein kinase C (PKC), leading to Vimentin phosphorylation and cytoskeletal reorganization. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Inhibits phosphatases, preventing Vimentin dephosphorylation, and thus maintains Vimentin in a phosphorylated, active state. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Acts as a phosphatase inhibitor, similar to Calyculin A, by preventing Vimentin dephosphorylation, leading to increased Vimentin phosphorylation. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Elevates intracellular cAMP levels, activating protein kinases that can phosphorylate Vimentin and promote its activation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Induces endoplasmic reticulum (ER) stress and can activate protein kinase C (PKC), leading to Vimentin phosphorylation and activation. | ||||||
Hydrogen Peroxide | 7722-84-1 | sc-203336 sc-203336A sc-203336B | 100 ml 500 ml 3.8 L | $31.00 $61.00 $95.00 | 28 | |
Activates various kinases, including MAP kinases, which can phosphorylate Vimentin and lead to its activation. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
Functions as a calcium ionophore, increasing intracellular calcium levels, which can activate calcium-dependent kinases that phosphorylate Vimentin. | ||||||