Vimentin Antibody (E-5): sc-373717

Vimentin Antibody (3CB2) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 419-438 of human vimentin, a 57 kDa intermediate filament protein crucial for cellular architecture and function in mesenchymal cells. Vimentin mouse monoclonal antibody (3CB2) recognizes an epitope within the vimentin immunogen sequence. The molecular architecture of vimentin reveals sophisticated organization: vimentin molecules form α-helical coiled-coil dimers that assemble into higher-order structures, creating a stable yet dynamic filament system. This complex network serves multiple essential functions within cells - vimentin filaments maintain cell shape, position organelles, and enable cells to withstand mechanical stress. While vimentin predominantly resides in the cytoplasm, vimentin redistributes to the perinuclear region during cell division and stress conditions, facilitating processes such as cell migration, division, and adhesion. Vimentin participates in several crucial protein partnerships that extend beyond structural support. One significant interaction occurs with integrin-linked kinase (ILK), a key mediator of integrin signaling that controls cell adhesion, migration, and survival. Through vimentin′s association with ILK, cells can effectively transmit extracellular signals to the cytoskeleton - a process particularly important during epithelial-mesenchymal transition (EMT), when cells must extensively remodel their architecture for cancer progression and metastasis. Another critical partnership exists between vimentin and plectin, a large cytolinker protein that connects vimentin filaments to other cytoskeletal components such as actin filaments and microtubules. This interconnected network provides enhanced structural integrity, allowing cells to better resist mechanical forces, especially in tissues subject to significant physical stress like muscle and connective tissues. These sophisticated molecular interactions highlight vimentin′s role not only as a structural protein but as a central coordinator of cellular mechanics and signaling.

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Vimentin Antibody (E-5) References:

1. Critical role of vimentin phosphorylation at Ser-56 by p21-activated kinase in vimentin cytoskeleton signaling.  |  Li, QF., et al. 2006. J Biol Chem. 281: 34716-24. PMID: 16990256
2. Vimentin supports mitochondrial morphology and organization.  |  Tang, HL., et al. 2008. Biochem J. 410: 141-6. PMID: 17983357
3. Effects of vimentin disruption on the mechanoresponses of articular chondrocyte.  |  Chen, C., et al. 2016. Biochem Biophys Res Commun. 469: 132-137. PMID: 26616052
4. Impact of Expression of Vimentin and Axl in Breast Cancer.  |  Tanaka, K., et al. 2016. Clin Breast Cancer. 16: 520-526.e2. PMID: 27506606
5. Vimentin Modulates Infectious Internalization of Human Papillomavirus 16 Pseudovirions.  |  Schäfer, G., et al. 2017. J Virol. 91: PMID: 28566373
6. Epithelial vimentin plays a functional role in mammary gland development.  |  Peuhu, E., et al. 2017. Development. 144: 4103-4113. PMID: 28947532
7. Proteomic Analysis Implicates Vimentin in Glioblastoma Cell Migration.  |  Nowicki, MO., et al. 2019. Cancers (Basel). 11: PMID: 30987208
8. Exogenous Vimentin Supplementation Transiently Affects Early Steps during HPV16 Pseudovirus Infection.  |  Carse, S., et al. 2021. Viruses. 13: PMID: 34960740
9. Vimentin immunoexpression and its prognostic activity in colon cancer among Caucasian patients.  |  Brzozowa-Zasada, M., et al. 2022. Prz Gastroenterol. 17: 123-129. PMID: 35664018
10. Extracellular Vimentin Alters Energy Metabolism And Induces Adipocyte Hypertrophy.  |  Park, JH., et al. 2024. Diabetes Metab J. 48: 215-230. PMID: 37750184

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vimentin Antibody (E-5) has 212 citations in a variety of scientific publications.

Primary antibodies like Anti-Vimentin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vimentin Antibody (E-5) Specifications

Alternate Product Name: Vimentin

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 419-438

Epitope Target: Vimentin

Epitope Species: human

Product Reactivity/Detection: Vimentin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 212 citations in various scientific publications

Available Conjugates/Forms for Vimentin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Vimentin antibody is Vimentin Antibody (V9): sc-6260.