VCP Antibody (D-9): sc-133212
- VCP Antibody (D-9) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 687-806 mapping at the C-terminus of VCP of human origin
- Anti-VCP Antibody (D-9) is recommended for detection of VCP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-VCP Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VCP Antibody (D-9) for WB and IHC(P) applications. These reagents are now offered in bundles with VCP Antibody (D-9) (see ordering information below).
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VCP Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Valosin Containing Protein (VCP) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). VCP (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Valosin Containing Protein, also known as TERA (transitional endoplasmic reticulum ATPase) or p97, plays a crucial role in cellular processes as a member of the AAA family of ATPases. VCP is primarily located in the cytoplasm and is essential for various cellular functions, including cell cycle regulation and membrane dynamics. VCP is particularly important for misfolded protein degradation and cellular component recycling, as VCP interacts with ubiquitinated substrates and the 26S proteasome, facilitating damaged protein removal. Proper functioning of VCP is vital for maintaining cellular homeostasis, and VCP dysregulation has been implicated in several diseases, including neurodegenerative disorders and cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VCP Antibody (D-9) References:
- Nucleocytoplasmic shuttling of valosin-containing protein (VCP/p97) regulated by its N domain and C-terminal region. | Song, C., et al. 2015. Biochim Biophys Acta. 1853: 222-32. PMID: 25447673
- Valosin-containing protein (VCP/p97) is capable of unfolding polyubiquitinated proteins through its ATPase domains. | Song, C., et al. 2015. Biochem Biophys Res Commun. 463: 453-7. PMID: 26043696
- Valosin-containing protein VCP/p97 is essential for the intracellular development of Leishmania and its survival under heat stress. | Guedes Aguiar, B., et al. 2018. Cell Microbiol. 20: e12867. PMID: 29895095
- Valosin-containing protein is associated with maintenance of meiotic arrest in mouse oocytes†. | Peng, H., et al. 2019. Biol Reprod. 100: 963-970. PMID: 30476006
- Valosin-containing Protein in Psoriasis: A Clinical and Immunohistochemical Study. | El Dein Mohamed, AS., et al. 2021. Appl Immunohistochem Mol Morphol. 29: e68-e72. PMID: 33979096
- Flavivirus recruits the valosin-containing protein-NPL4 complex to induce stress granule disassembly for efficient viral genome replication. | Arakawa, M., et al. 2022. J Biol Chem. 298: 101597. PMID: 35063505
- Valosin-containing protein-regulated endoplasmic reticulum stress causes NOD2-dependent inflammatory responses. | Ghalandary, M., et al. 2022. Sci Rep. 12: 3906. PMID: 35273242
- Valosin Containing Protein as a Specific Biomarker for Predicting the Development of Acute Coronary Syndrome and Its Complication. | Xu, C., et al. 2022. Front Cardiovasc Med. 9: 803532. PMID: 35369356
- Valosin-containing protein Asp395Gly mutation in a patient with frontotemporal dementia: a case report. | Kobayashi, R., et al. 2022. BMC Neurol. 22: 406. PMID: 36329418
- Valosin-containing protein (VCP/p97) is prognostically unfavorable in pediatric AML, and negatively correlates with unfolded protein response proteins IRE1 and GRP78: A report from the Children's Oncology Group. | Hoff, FW., et al. 2023. Proteomics Clin Appl. 17: e2200109. PMID: 37287368
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VCP Antibody (D-9) | sc-133212 | 200 µg/ml | $316.00 | |||
VCP Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-528770 | 200 µg Ab; 10 µg BP | $354.00 | |||
VCP Antibody (D-9): m-IgGκ BP-HRP Bundle | sc-521276 | 200 µg Ab, 40 µg BP | $354.00 | |||
VCP Antibody (D-9): m-IgG1 BP-HRP Bundle | sc-543119 | 200 µg Ab; 20 µg BP | $354.00 | |||
VCP Antibody (D-9) AC | sc-133212 AC | 500 µg/ml, 25% agarose | $416.00 | |||
VCP Antibody (D-9) HRP | sc-133212 HRP | 200 µg/ml | $316.00 | |||
VCP Antibody (D-9) FITC | sc-133212 FITC | 200 µg/ml | $330.00 | |||
VCP Antibody (D-9) PE | sc-133212 PE | 200 µg/ml | $343.00 | |||
VCP Antibody (D-9) Alexa Fluor® 488 | sc-133212 AF488 | 200 µg/ml | $357.00 | |||
VCP Antibody (D-9) Alexa Fluor® 546 | sc-133212 AF546 | 200 µg/ml | $357.00 | |||
VCP Antibody (D-9) Alexa Fluor® 594 | sc-133212 AF594 | 200 µg/ml | $357.00 | |||
VCP Antibody (D-9) Alexa Fluor® 647 | sc-133212 AF647 | 200 µg/ml | $357.00 | |||
VCP Antibody (D-9) Alexa Fluor® 680 | sc-133212 AF680 | 200 µg/ml | $357.00 | |||
VCP Antibody (D-9) Alexa Fluor® 790 | sc-133212 AF790 | 200 µg/ml | $357.00 |