VCP Antibody (D-9) recommended for detection of VCP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

VCP Antibody (D-9): sc-133212

VCP Antibody (D-9) is rated 4.9 out of 5 by 8.
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Datasheets

    • VCP Antibody (D-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 687-806 mapping at the C-terminus of VCP of human origin
    • Anti-VCP Antibody (D-9) is recommended for detection of VCP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • Anti-VCP Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VCP (D-9): sc-133212.

I am using VCP (D-9): sc-133212 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from 效果不错 我1月22日收到该产品,组织蛋白进行Wb效果不错,特异性很好,建议一抗1:200,使用脱脂牛奶封闭。
Date published: 2018-03-23
Rated 5 out of 5 by from VCP antibody (D-9) I requested for a free sample of anti-VCP antibody and I am glad to mention that the antibody really worked very good for immunoblotting. I could get clean strong signal with the antibody. I would recommend using that antibody for experiments.
Date published: 2017-04-21
Rated 5 out of 5 by from useful I diluted the VCP antibody in 1:500 for three times , it impressed me for its effective and clear bind
Date published: 2017-03-27
Rated 5 out of 5 by from Very good antibody VCP antibody is used to do western blot,the result is very good.
Date published: 2017-03-07
Rated 5 out of 5 by from performed well Antibody performed well in a difficult application requiring a very clean, efficient IP: the search for a rare binding partner (CFTR) to a very abundant protein (VCP).
Date published: 2017-01-27
Rated 5 out of 5 by from Goog probe I use anti-VCP at a dilution of 1:1000/1:5000 in TBST 1hr and is enough for an excellent western signal blot.Loading 5 μg protein isenough.
Date published: 2016-11-24
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclear Produced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Satisfactory Western Blot data of VCP expression Satisfactory Western Blot data of VCP expression in non-transfected 293T, human VCP transfected 293T, HeLa, NIH/3T3 and PC-12 whole cell lysates. -SCBT QC
Date published: 2013-12-19
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