VCP Antibody (5) recommended for detection of VCP of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)

VCP Antibody (5): sc-57492

VCP Antibody (5) is rated 4.8 out of 5 by 10.
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Datasheets

    • Anti-VCP Antibody (5) is a mouse monoclonal IgG2b (kappa light chain) VCP antibody provided at 200 µg/ml
    • raised against amino acids 792-806 of VCP of human origin
    • Anti-VCP Antibody (5) is recommended for detection of VCP of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)
    • Anti-VCP Antibody (5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VCP (5): sc-57492.
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I am using VCP (5): sc-57492 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect for ICC Funciona excelentemente para ICC de fibroblastos primarios usandolo hasta 1:500 en PBST (0.1% Triton) + 10% Serum 1h RT u O/N 4ºC.
Date published: 2020-02-19
Rated 5 out of 5 by from Works for both IF and Western blot Brought this antibody 6 months ago. It works for both western blot and IF staining very well.
Date published: 2019-09-05
Rated 5 out of 5 by from Best VCP Antibody ever! Very clean and very specific! We use it to detect endogenous protein and it works great at 1:1000 loading very little lysate (10ug or less)
Date published: 2018-01-31
Rated 5 out of 5 by from Works very well on mouse liver WB Works very well on mouse liver lysates western blotting. very clean bands.
Date published: 2017-10-10
Rated 5 out of 5 by from Great for WB! Consistent, clean and strong bands seen across multiple whole cell lysate samples in Western Blot.
Date published: 2017-01-23
Rated 5 out of 5 by from good Abs this Abs was good for IP both on human and mouse cells
Date published: 2016-11-11
Rated 5 out of 5 by from Excellent Antibody I used this antibody on my Westerns and it worked very well.
Date published: 2016-10-31
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-05-13
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