VCP Antibody (5): sc-57492

VCP Antibody (5) is a mouse monoclonal IgG2b kappa light chain antibody that detects VCP protein of mouse, rat, human, and porcine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-VCP antibody (5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Valosin-containing protein (VCP), also known as TERA (transitional endoplasmic reticulum ATPase) or p97, is a crucial member of the AAA family of ATPases, which play significant roles in diverse cellular processes. VCP (5) monoclonal antibody recognizes a protein involved in regulating cell cycle and membrane dynamics, essential for extracting misfolded proteins from the endoplasmic reticulum and their subsequent degradation. This function maintains cellular homeostasis and prevents damaged protein accumulation, which can lead to various diseases, including neurodegenerative disorders. VCP (5) antibody targets a protein that interacts with ubiquitinated IκB-α and the 26S proteasome, highlighting its role in the proteasome-mediated degradation pathway and emphasizing its importance in cellular regulation and signaling.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

VCP Antibody (5) References:

1. Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1.  |  Zhang, SH., et al. 1999. J Biol Chem. 274: 17806-12. PMID: 10364224
2. VCP, the mammalian homolog of cdc48, is tyrosine phosphorylated in response to T cell antigen receptor activation.  |  Egerton, M., et al. 1992. EMBO J. 11: 3533-40. PMID: 1382975
3. Physical and functional interaction between Dorfin and Valosin-containing protein that are colocalized in ubiquitylated inclusions in neurodegenerative disorders.  |  Ishigaki, S., et al. 2004. J Biol Chem. 279: 51376-85. PMID: 15456787
4. VCP/p97 regulates Beclin-1-dependent autophagy initiation.  |  Hill, SM., et al. 2021. Nat Chem Biol. 17: 448-455. PMID: 33510452
5. Therapeutic developments for valosin-containing protein mediated multisystem proteinopathy.  |  Boock, V., et al. 2023. Curr Opin Neurol. 36: 432-440. PMID: 37678339
6. Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis.  |  Hao, J., et al. 2023. Cell Rep. 42: 113449. PMID: 37967009
7. XAF1 promotes colorectal cancer metastasis via VCP-RNF114-JUP axis.  |  Xia, J., et al. 2024. J Cell Biol. 223: PMID: 38095639
8. A 200-amino acid ATPase module in search of a basic function.  |  Confalonieri, F. and Duguet, M. 1995. Bioessays. 17: 639-50. PMID: 7646486
9. Biochemical characterization of valosin-containing protein, a protein tyrosine kinase substrate in hematopoietic cells.  |  Egerton, M. and Samelson, LE. 1994. J Biol Chem. 269: 11435-41. PMID: 8157674
10. Chromosome localization of human genes for clathrin adaptor polypeptides AP2 beta and AP50 and the clathrin-binding protein, VCP.  |  Druck, T., et al. 1995. Genomics. 30: 94-7. PMID: 8595912
11. Tyrosine phosphorylation regulates cell cycle-dependent nuclear localization of Cdc48p.  |  Madeo, F., et al. 1998. Mol Biol Cell. 9: 131-41. PMID: 9436996
12. Involvement of valosin-containing protein, an ATPase Co-purified with IkappaBalpha and 26 S proteasome, in ubiquitin-proteasome-mediated degradation of IkappaBalpha.  |  Dai, RM., et al. 1998. J Biol Chem. 273: 3562-73. PMID: 9452483

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VCP Antibody (5) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-VCP Antibody (5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VCP Antibody (5) Specifications

Alternate Product Name: Valosin Containing Protein

Product Clone Name: 5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 792-806

Epitope Target: VCP

Epitope Species: human

Product Reactivity/Detection: VCP

Species Reactivity/Detection: mouse, rat, human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for VCP Antibody (5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin