V-ATPase G1 Antibody (D-5): sc-25333
- V-ATPase G1 Antibody (D-5) is a mouse monoclonal IgG1 κ V-ATPase G1 antibody, cited in 10 publications, provided at 200 µg/ml
- raised against amino acids 39-118 of V-ATPase G1 of human origin
- V-ATPase G1 Antibody (D-5) is recommended for detection of V-ATPase G1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-V-ATPase G1 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase G1 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase G1 Antibody (D-5) (see ordering information below).
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V-ATPase G1 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects V-ATPase G1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-V-ATPase G1 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. V-ATPase G1 plays a crucial role in the acidification of eukaryotic intracellular organelles, which is essential for processes such as protein degradation, nutrient storage, and cellular pH regulation. V-ATPase G1 functions as part of the vacuolar-type H+-ATPase complex, which consists of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that facilitates proton translocation. The V1 domain is composed of nine subunits (A-H), while the V0 domain contains five subunits (a, d, c, c′, and c"). V-ATPase G1 is involved in the assembly and stability of the V-ATPase complex, and may interact with other proteins, such as the pore-forming a1C-subunit of L-type calcium channels, to ensure proper membrane localization and function. The genes encoding G1 and G2 V-ATPase subunits are located on chromosomes 9q33.1 and 6p21.3, respectively, highlighting their genetic significance in cellular physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
V-ATPase G1 Antibody (D-5) References:
- A new member of the Ig superfamily and a V-ATPase G subunit are among the predicted products of novel genes close to the TNF locus in the human MHC. | Neville, MJ. and Campbell, RD. 1999. J Immunol. 162: 4745-54. PMID: 10202016
- Mutational analysis of subunit G (Vma10p) of the yeast vacuolar H+-ATPase. | Charsky, CM., et al. 2000. J Biol Chem. 275: 37232-9. PMID: 10969085
- Association of L-type calcium channels with a vacuolar H(+)-ATPase G2 subunit. | Gao, T. and Hosey, MM. 2000. Biochem Biophys Res Commun. 277: 611-6. PMID: 11062002
- The vacuolar (H+)-ATPases--nature's most versatile proton pumps. | Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
- Molecular cloning and characterization of novel tissue-specific isoforms of the human vacuolar H(+)-ATPase C, G and d subunits, and their evaluation in autosomal recessive distal renal tubular acidosis. | Smith, AN., et al. 2002. Gene. 297: 169-77. PMID: 12384298
- Defined sites of interaction between subunits E (Vma4p), C (Vma5p), and G (Vma10p) within the stator structure of the vacuolar H+-ATPase. | Jones, RP., et al. 2005. Biochemistry. 44: 3933-41. PMID: 15751969
- Transcript level regulation of the vacuolar H(+)-ATPase subunit isoforms VHA-a, VHA-E and VHA-G in Arabidopsis thaliana. | Hanitzsch, M., et al. 2007. Mol Membr Biol. 24: 507-18. PMID: 17710654
- The intriguing evolution of the 'b' and 'G' subunits in F-type and V-type ATPases: isolation of the vma-10 gene from Neurospora crassa. | Hunt, IE. and Bowman, BJ. 1997. J Bioenerg Biomembr. 29: 533-40. PMID: 9559854
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
V-ATPase G1 Antibody (D-5) | sc-25333 | 200 µg/ml | $316.00 | |||
V-ATPase G1 Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-528406 | 200 µg Ab; 10 µg BP | $354.00 | |||
V-ATPase G1 Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-520779 | 200 µg Ab, 40 µg BP | $354.00 | |||
V-ATPase G1 Antibody (D-5): m-IgG1 BP-HRP Bundle | sc-542918 | 200 µg Ab; 20 µg BP | $354.00 | |||
V-ATPase G1 Antibody (D-5) AC | sc-25333 AC | 500 µg/ml, 25% agarose | $416.00 | |||
V-ATPase G1 Antibody (D-5) HRP | sc-25333 HRP | 200 µg/ml | $316.00 | |||
V-ATPase G1 Antibody (D-5) FITC | sc-25333 FITC | 200 µg/ml | $330.00 | |||
V-ATPase G1 Antibody (D-5) PE | sc-25333 PE | 200 µg/ml | $343.00 | |||
V-ATPase G1 Antibody (D-5) Alexa Fluor® 488 | sc-25333 AF488 | 200 µg/ml | $357.00 | |||
V-ATPase G1 Antibody (D-5) Alexa Fluor® 546 | sc-25333 AF546 | 200 µg/ml | $357.00 | |||
V-ATPase G1 Antibody (D-5) Alexa Fluor® 594 | sc-25333 AF594 | 200 µg/ml | $357.00 | |||
V-ATPase G1 Antibody (D-5) Alexa Fluor® 647 | sc-25333 AF647 | 200 µg/ml | $357.00 | |||
V-ATPase G1 Antibody (D-5) Alexa Fluor® 680 | sc-25333 AF680 | 200 µg/ml | $357.00 | |||
V-ATPase G1 Antibody (D-5) Alexa Fluor® 790 | sc-25333 AF790 | 200 µg/ml | $357.00 |