V-ATPase B1/2 Antibody (D-4): sc-271832

V-ATPase B1/2 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects V-ATPase B1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). V-ATPase B1/2 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The vacuolar-type H+-ATPase (V-ATPase) is a crucial multisubunit enzyme responsible for the acidification of eukaryotic intracellular organelles, which is vital for processes such as protein degradation, nutrient absorption, and maintaining cellular homeostasis. The V-ATPase operates through a rotary mechanism, consisting of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that facilitates proton translocation. The V1 domain comprises nine subunits (A-H), while the V0 domain consists of five subunits (a, d, c, c′, and c"). Notably, V-ATPase B1 isoform plays a critical role in the inner ear, where V-ATPase B1 is essential for proton secretion, maintaining the proper pH of endolymph, and ensuring normal auditory function. Mutations in the gene encoding V-ATPase B1 isoform, located on chromosome 2cen-q13, have been linked to distal renal tubular acidosis associated with sensorineural deafness. Meanwhile, V-ATPase B2 isoform, which is expressed in the kidney and osteoclasts, is encoded by a gene mapped to chromosome 8p22-p21. The diverse applications and specificity of V-ATPase B1/2 (D-4) monoclonal antibody make V-ATPase B1/2 (D-4) antibody an invaluable tool for researchers studying the physiological and pathological roles of V-ATPase in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

V-ATPase B1/2 Antibody (D-4) References:

1. The lysosomal v-ATPase-Ragulator complex is a common activator for AMPK and mTORC1, acting as a switch between catabolism and anabolism.  |  Zhang, CS., et al. 2014. Cell Metab. 20: 526-40. PMID: 25002183
2. STING induces LC3B lipidation onto single-membrane vesicles via the V-ATPase and ATG16L1-WD40 domain.  |  Fischer, TD., et al. 2020. J Cell Biol. 219: PMID: 33201170
3. Overexpression of V-ATPase B2 attenuates lung injury/fibrosis by stabilizing lysosomal membrane permeabilization and increasing collagen degradation.  |  Lee, JU., et al. 2022. Exp Mol Med. 54: 662-672. PMID: 35624153
4. Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly.  |  Ratto, E., et al. 2022. Nat Commun. 13: 4848. PMID: 35977928
5. ATP6V0C variants impair V-ATPase function causing a neurodevelopmental disorder often associated with epilepsy.  |  Mattison, KA., et al. 2023. Brain. 146: 1357-1372. PMID: 36074901
6. Schisandrol A protects AGEs-induced neuronal cells death by allosterically targeting ATP6V0d1 subunit of V-ATPase.  |  Zhou, X., et al. 2022. Acta Pharm Sin B. 12: 3843-3860. PMID: 36213534
7. V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation.  |  Sun, Y., et al. 2023. Dev Cell. 58: 2761-2775.e5. PMID: 37922908
8. Structure and topography of the synaptic V-ATPase-synaptophysin complex.  |  Wang, C., et al. 2024. Nature. 631: 899-904. PMID: 38838737
9. The different roles of V-ATPase a subunits in phagocytosis/endocytosis and autophagy.  |  Chen, Q., et al. 2024. Autophagy. 20: 2297-2313. PMID: 38873931
10. High-resolution electron cryomicroscopy of V-ATPase in native synaptic vesicles.  |  Coupland, CE., et al. 2024. Science. 385: 168-174. PMID: 38900912

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V-ATPase B1/2 Antibody (D-4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-V-ATPase B1/2 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V-ATPase B1/2 Antibody (D-4) Specifications

Alternate Product Name: ATP6V1B1 / ATP6V1B2

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 103-131

Epitope Target: V-ATPase B1/2

Epitope Species: human

Product Reactivity/Detection: V-ATPase B1 and V-ATPase B2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for V-ATPase B1/2 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred V-ATPase B1/2 antibody is V-ATPase B1/2 Antibody (F-6): sc-55544.