V-ATPase B1/2 Antibody (F-6): sc-55544

V-ATPase B1/2 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects V-ATPase B1/2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). V-ATPase B1/2 (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The vacuolar-type H+-ATPase (V-ATPase) is a crucial multisubunit enzyme that plays a vital role in acidifying eukaryotic intracellular organelles, essential for protein sorting, nutrient uptake, and cellular signaling. V-ATPase operates through a rotary mechanism, consisting of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that facilitates proton translocation. The V1 domain comprises nine subunits (A-H), while the V0 domain consists of five subunits (a, d, c, c′, and c"). V-ATPase B1 isoform is particularly important in the inner ear, where V-ATPase B1 regulates proton secretion to maintain proper pH of endolymph, ensuring normal auditory function. Mutations in the gene encoding V-ATPase B1 isoform, located on chromosome 2q13.1, have been linked to distal renal tubular acidosis and sensorineural deafness, highlighting V-ATPase B1′s critical role in both renal and auditory health. V-ATPase B2 isoform is predominantly expressed in the kidney and osteoclasts, with V-ATPase B2′s gene mapping to chromosome 8p22-p21, further emphasizing the diverse physiological functions of V-ATPase in different tissues.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

V-ATPase B1/2 Antibody (F-6) References:

1. The vacuolar (H+)-ATPases--nature's most versatile proton pumps.  |  Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
2. Selectively amplified expression of an isoform of the vacuolar H(+)-ATPase 56-kilodalton subunit in renal intercalated cells.  |  Nelson, RD., et al. 1992. Proc Natl Acad Sci U S A. 89: 3541-5. PMID: 1373501
3. An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H(+)-ATPase.  |  Bernasconi, P., et al. 1990. J Biol Chem. 265: 17428-31. PMID: 2145275
4. Disorders of lysosomal acidification-The emerging role of v-ATPase in aging and neurodegenerative disease.  |  Colacurcio, DJ. and Nixon, RA. 2016. Ageing Res Rev. 32: 75-88. PMID: 27197071
5. Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes.  |  Abu-Remaileh, M., et al. 2017. Science. 358: 807-813. PMID: 29074583
6. A Bacterial Effector Reveals the V-ATPase-ATG16L1 Axis that Initiates Xenophagy.  |  Xu, Y., et al. 2019. Cell. 178: 552-566.e20. PMID: 31327526
7. Structure of V-ATPase from the mammalian brain.  |  Abbas, YM., et al. 2020. Science. 367: 1240-1246. PMID: 32165585
8. Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.  |  Wang, L., et al. 2020. Mol Cell. 80: 501-511.e3. PMID: 33065002
9. V-ATPase is a universal regulator of LC3-associated phagocytosis and non-canonical autophagy.  |  Hooper, KM., et al. 2022. J Cell Biol. 221: PMID: 35511089
10. Osteoclasts express the B2 isoform of vacuolar H(+)-ATPase intracellularly and on their plasma membranes.  |  Lee, BS., et al. 1996. Am J Physiol. 270: C382-8. PMID: 8772466
11. Mutations in the gene encoding B1 subunit of H+-ATPase cause renal tubular acidosis with sensorineural deafness.  |  Karet, FE., et al. 1999. Nat Genet. 21: 84-90. PMID: 9916796

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V-ATPase B1/2 Antibody (F-6) has 46 citations in a variety of scientific publications.

Primary antibodies like Anti-V-ATPase B1/2 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V-ATPase B1/2 Antibody (F-6) Specifications

Alternate Product Name: ATP6V1B1 / ATP6V1B2

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 334-513

Epitope Target: V-ATPase B1/2

Epitope Species: human

Product Reactivity/Detection: V-ATPase B and V-ATPase B2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 46 citations in various scientific publications

Available Conjugates/Forms for V-ATPase B1/2 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin