V-ATPase A1 Antibody (E-8): sc-374475

V-ATPase A1 Antibody (E-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects V-ATPase A1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). V-ATPase A1 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. V-ATPase A1 protein plays a crucial role in cellular processes by functioning as a proton pump that regulates pH and ion homeostasis within cells. V-ATPase A1 is primarily located in clathrin-coated vesicles and osteoclasts, where V-ATPase A1 is essential for acidification of intracellular compartments, facilitating processes such as protein degradation and nutrient absorption. V-ATPase A1 consists of two main domains: a hydrophilic amino-terminus rich in charged residues and a hydrophobic carboxy-terminus that features multiple transmembrane regions. This structural arrangement enables coupling of ATP hydrolysis to proton translocation across membranes. Inactivation of osteoclast-specific vacuolar proton ATPase subunit leads to impaired bone resorption, contributing to osteopetrosis in mutant mice. V-ATPase A1 co-localizes with late endosomal marker Rab7, highlighting V-ATPase A1′s importance in vacuole formation through selective swelling of late endosomes, which is critical for maintaining cellular homeostasis and function.

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V-ATPase A1 Antibody (E-8) References:

1. The gene encoding the mouse homologue of the human osteoclast-specific 116-kDa V-ATPase subunit bears a deletion in osteosclerotic (oc/oc) mutants.  |  Scimeca, JC., et al. 2000. Bone. 26: 207-13. PMID: 10709991
2. Salicylihalamide A inhibits the V0 sector of the V-ATPase through a mechanism distinct from bafilomycin A1.  |  Xie, XS., et al. 2004. J Biol Chem. 279: 19755-63. PMID: 14998996
3. Structure of the 116-kDa polypeptide of the clathrin-coated vesicle/synaptic vesicle proton pump.  |  Perin, MS., et al. 1991. J Biol Chem. 266: 3877-81. PMID: 1704894
4. Bafilomycin A1 disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion.  |  Mauvezin, C. and Neufeld, TP. 2015. Autophagy. 11: 1437-8. PMID: 26156798
5. Modulation of V-ATPase by βA3/A1-Crystallin in Retinal Pigment Epithelial Cells.  |  Valapala, M., et al. 2016. Adv Exp Med Biol. 854: 779-84. PMID: 26427489
6. V-ATPase (Vacuolar ATPase) Activity Required for ABCA1 (ATP-Binding Cassette Protein A1)-Mediated Cholesterol Efflux.  |  Lorkowski, SW., et al. 2018. Arterioscler Thromb Vasc Biol. 38: 2615-2625. PMID: 30354238
7. Proton pumping V-ATPase inhibitor bafilomycin A1 affects Rab7 lysosomal localization and abolishes anterograde trafficking of osteoclast secretory lysosomes.  |  Matsumoto, N. and Nakanishi-Matsui, M. 2019. Biochem Biophys Res Commun. 510: 421-426. PMID: 30717974
8. Molecular basis of V-ATPase inhibition by bafilomycin A1.  |  Wang, R., et al. 2021. Nat Commun. 12: 1782. PMID: 33741963
9. Structural and functional understanding of disease-associated mutations in V-ATPase subunit a1 and other isoforms.  |  Indrawinata, K., et al. 2023. Front Mol Neurosci. 16: 1135015. PMID: 37465367
10. Alternative mRNA splicing generates tissue-specific isoforms of 116-kDa polypeptide of vacuolar proton pump.  |  Peng, SB., et al. 1994. J Biol Chem. 269: 17262-6. PMID: 8006034
11. The vacuolar ATPase proton pump is present on intracellular vacuoles induced by Helicobacter pylori.  |  Papini, E., et al. 1996. J Med Microbiol. 45: 84-9. PMID: 8683556
12. Identification and reconstitution of an isoform of the 116-kDa subunit of the vacuolar proton translocating ATPase.  |  Peng, SB., et al. 1999. J Biol Chem. 274: 2549-55. PMID: 9891027

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V-ATPase A1 Antibody (E-8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-V-ATPase A1 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V-ATPase A1 Antibody (E-8) Specifications

Alternate Product Name: ATP6V0A1

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 71-210

Epitope Target: V-ATPase A1

Epitope Species: human

Product Reactivity/Detection: V-ATPase A1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine, porcine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for V-ATPase A1 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin