Anti-USP25 Antibody (C-5) recommended for detection of USP25 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-USP25 Antibody (C-5): sc-398414

Anti-USP25 Antibody (C-5) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-USP25 Antibody (C-5) is a mouse monoclonal IgG1 κ USP25 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 851-990 mapping near the C-terminus of USP25 of human origin
    • Anti-USP25 Antibody (C-5) is recommended for detection of USP25 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-USP25 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of USP25 (C-5): sc-398414.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for USP25 Antibody (C-5) for WB applications. These reagents are now offered in bundles with USP25 Antibody (C-5) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with USP25 (C-5): sc-398414 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 非常好用,WB实验结果显示无杂带。 我一个半月前订个该抗体试用装,使用效果不错,无杂带,相对较容易显影。只是试用装量少,只够做一次WB。
Date published: 2017-08-15
Rated 5 out of 5 by from Excellent antibody! The USP25 Antibody (C-5): sc-398414 provides good staining pattern for IF, showing cytoplasmic localization.
Date published: 2017-01-19
Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmic Excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Great Western Blot data of USP25 expression Great Western Blot data of USP25 expression in NTERA-2 cl.D1 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2014-03-04
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