USP14 Antibody (F-4): sc-398009

USP14 Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects USP14 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). USP14 Antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USP14, also known as ubiquitin-specific peptidase 14, plays a crucial role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. Located primarily in the cytoplasm, USP14 functions as a homodimer and removes ubiquitin moieties from ubiquitin-conjugated proteins, thus preventing their degradation by the proteasome. This activity is vital for various cellular processes, including signal transduction and cell cycle regulation. Defects in USP14 can lead to severe developmental issues in mice, highlighting USP14′s importance in early growth and development. Alternative splicing of USP14 results in multiple isoforms that may have distinct regulatory roles within the cell. With diverse applications and critical biological functions, anti-USP14 antibody (F-4) serves as an invaluable tool for researchers studying protein regulation and degradation pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

USP14 Antibody (F-4) References:

1. USP14 maintains HIF1-α stabilization via its deubiquitination activity in hepatocellular carcinoma.  |  Lv, C., et al. 2021. Cell Death Dis. 12: 803. PMID: 34420039
2. USP14-regulated allostery of the human proteasome by time-resolved cryo-EM.  |  Zhang, S., et al. 2022. Nature. 605: 567-574. PMID: 35477760
3. MFG-E8 stabilized by deubiquitinase USP14 suppresses cigarette smoke-induced ferroptosis in bronchial epithelial cells.  |  Cui, Y., et al. 2023. Cell Death Dis. 14: 2. PMID: 36596780
4. USP14 promotes colorectal cancer progression by targeting JNK for stabilization.  |  Du, XH., et al. 2023. Cell Death Dis. 14: 56. PMID: 36693850
5. SDC2 Stabilization by USP14 Promotes Gastric Cancer Progression through Co-option of PDK1.  |  You, L., et al. 2023. Int J Biol Sci. 19: 3483-3498. PMID: 37496999
6. Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells.  |  Ding, W., et al. 2023. Acta Pharmacol Sin. 44: 2537-2548. PMID: 37528233
7. USP14 exhibits high expression levels in hepatocellular carcinoma and plays a crucial role in promoting the growth of liver cancer cells through the HK2/AKT/P62 axis.  |  Zhang, N., et al. 2024. BMC Cancer. 24: 237. PMID: 38383348
8. USP14 increases the sensitivity of retinoblastoma to cisplatin by mediating the ferroptosis.  |  Liu, H., et al. 2024. Naunyn Schmiedebergs Arch Pharmacol. 397: 8671-8680. PMID: 38819674
9. USP14 deficiency inhibits neointima formation following vascular injury via degradation of Skp2 protein.  |  Xia, X., et al. 2024. Cell Death Discov. 10: 295. PMID: 38909015
10. USP14 inhibition promotes DNA damage repair and represses ovarian granulosa cell senescence in premature ovarian insufficiency.  |  Ma, LZ., et al. 2024. J Transl Med. 22: 834. PMID: 39261935

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP14 Antibody (F-4) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-USP14 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP14 Antibody (F-4) Specifications

Alternate Product Name: USP14

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 195-494

Epitope Target: USP14

Epitope Species: human

Product Reactivity/Detection: USP14

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for USP14 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred USP14 antibody is USP14 Antibody (6E6): sc-100630.