USP14 Antibody (6E6): sc-100630

USP14 Antibody (6E6) is a mouse monoclonal IgG2a kappa light chain antibody that detects USP14 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-USP14 antibody (6E6) is available in a non-conjugated form, allowing for versatile experimental use. USP14, also known as ubiquitin specific peptidase 14, is a crucial cytoplasmic protein that plays a significant role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. USP14 exists as a homodimer and functions primarily to remove ubiquitin moieties from ubiquitin-conjugated proteins, thus preventing their degradation by the proteasome. USP14′s importance is underscored by its involvement in various cellular processes, including signal transduction and cell cycle regulation. Notably, defects in USP14 can lead to severe developmental issues in mice, highlighting USP14′s critical role in early development. Additionally, USP14 undergoes post-translational modifications that can influence activity and interactions with other proteins, further emphasizing USP14′s importance in cellular regulation and signaling pathways. Multiple isoforms of USP14 arise from alternative splicing, contributing to the complexity of regulatory functions within the cell.

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USP14 Antibody (6E6) References:

1. A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14.  |  Borodovsky, A., et al. 2001. EMBO J. 20: 5187-96. PMID: 11566882
2. Synaptic defects in ataxia mice result from a mutation in Usp14, encoding a ubiquitin-specific protease.  |  Wilson, SM., et al. 2002. Nat Genet. 32: 420-5. PMID: 12368914
3. Loss of Usp14 results in reduced levels of ubiquitin in ataxia mice.  |  Anderson, C., et al. 2005. J Neurochem. 95: 724-31. PMID: 16190881
4. Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14.  |  Hu, M., et al. 2005. EMBO J. 24: 3747-56. PMID: 16211010
5. Ubiquitin-specific protease 14 expression in colorectal cancer is associated with liver and lymph node metastases.  |  Shinji, S., et al. 2006. Oncol Rep. 15: 539-43. PMID: 16465409
6. Transgenic rescue of ataxia mice with neuronal-specific expression of ubiquitin-specific protease 14.  |  Crimmins, S., et al. 2006. J Neurosci. 26: 11423-31. PMID: 17079671
7. Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome.  |  Koulich, E., et al. 2008. Mol Biol Cell. 19: 1072-82. PMID: 18162577
8. Proteome-wide analysis of USP14 substrates revealed its role in hepatosteatosis via stabilization of FASN.  |  Liu, B., et al. 2018. Nat Commun. 9: 4770. PMID: 30425250
9. Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.  |  Ming, SL., et al. 2022. Autophagy. 18: 1801-1821. PMID: 34822318
10. USP14 promotes tryptophan metabolism and immune suppression by stabilizing IDO1 in colorectal cancer.  |  Shi, D., et al. 2022. Nat Commun. 13: 5644. PMID: 36163134
11. Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit.  |  Deshpande, KL., et al. 1996. Arch Biochem Biophys. 326: 1-7. PMID: 8579355
12. Deubiquitinating enzymes: a new class of biological regulators.  |  D'Andrea, A. and Pellman, D. 1998. Crit Rev Biochem Mol Biol. 33: 337-52. PMID: 9827704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP14 Antibody (6E6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-USP14 Antibody (6E6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP14 Antibody (6E6) Specifications

Alternate Product Name: USP14

Product Clone Name: 6E6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 395-495

Epitope Target: USP14

Epitope Species: human

Product Reactivity/Detection: USP14

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for USP14 Antibody (6E6): N/A