USE1 Antibody (D-1): sc-398316
- USE1 Antibody (D-1) is a mouse monoclonal IgG1 κ USE1 antibody provided at 200 µg/ml
- raised against amino acids 1-78 mapping at the N-terminus of USE1 of human origin
- USE1 Antibody (D-1) is recommended for detection of USE1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-USE1 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for USE1 Antibody (D-1) for WB applications. These reagents are now offered in bundles with USE1 Antibody (D-1) (see ordering information below).
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USE1 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects USE1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). USE1 (D-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USE1, also known as unconventional SNARE in the ER 1, plays a crucial role in the vesicular transport system of eukaryotic cells, specifically in the Golgi apparatus where USE1 facilitates the delivery of newly synthesized proteins from the endoplasmic reticulum to their final destinations. This process is vital for maintaining cellular function and ensuring that proteins reach the correct membrane compartments, which is essential for proper cell signaling and function. USE1 is a single-pass type IV membrane protein that interacts with other proteins such as ZW10, RINT-1, and Syntaxin 18 to form a larger complex, and USE1 is localized to the endoplasmic reticulum membrane. USE1 consists of 259 amino acids and has three known isoforms resulting from alternative splicing events, highlighting USE1′s complexity and potential for diverse functional roles within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
USE1 Antibody (D-1) References:
- Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways. | Matsuda, A., et al. 2003. Oncogene. 22: 3307-18. PMID: 12761501
- A SNARE required for retrograde transport to the endoplasmic reticulum. | Burri, L., et al. 2003. Proc Natl Acad Sci U S A. 100: 9873-7. PMID: 12893879
- Yeast functional analysis: identification of two essential genes involved in ER to Golgi trafficking. | Belgareh-Touzé, N., et al. 2003. Traffic. 4: 607-17. PMID: 12911815
- Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi. | Hirose, H., et al. 2004. EMBO J. 23: 1267-78. PMID: 15029241
- Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion. | Nakajima, K., et al. 2004. EMBO J. 23: 3216-26. PMID: 15272311
- Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system. | Okumura, AJ., et al. 2006. J Biol Chem. 281: 4495-506. PMID: 16354670
- Synaptic vesicle fusion. | Rizo, J. and Rosenmund, C. 2008. Nat Struct Mol Biol. 15: 665-74. PMID: 18618940
- SNAREs and membrane fusion in the Golgi apparatus. | Nichols, BJ. and Pelham, HR. 1998. Biochim Biophys Acta. 1404: 9-31. PMID: 9714710
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USE1 Antibody (D-1) | sc-398316 | 200 µg/ml | $322.00 | |||
USE1 Antibody (D-1): m-IgG Fc BP-HRP Bundle | sc-530756 | 200 µg Ab; 10 µg BP | $361.00 | |||
USE1 Antibody (D-1): m-IgGκ BP-HRP Bundle | sc-524291 | 200 µg Ab, 40 µg BP | $361.00 | |||
USE1 Antibody (D-1): m-IgG1 BP-HRP Bundle | sc-544237 | 200 µg Ab; 20 µg BP | $361.00 | |||
USE1 Antibody (D-1) AC | sc-398316 AC | 500 µg/ml, 25% agarose | $424.00 | |||
USE1 Antibody (D-1) HRP | sc-398316 HRP | 200 µg/ml | $322.00 | |||
USE1 Antibody (D-1) FITC | sc-398316 FITC | 200 µg/ml | $336.00 | |||
USE1 Antibody (D-1) PE | sc-398316 PE | 200 µg/ml | $349.00 | |||
USE1 Antibody (D-1) Alexa Fluor® 488 | sc-398316 AF488 | 200 µg/ml | $364.00 | |||
USE1 Antibody (D-1) Alexa Fluor® 546 | sc-398316 AF546 | 200 µg/ml | $364.00 | |||
USE1 Antibody (D-1) Alexa Fluor® 594 | sc-398316 AF594 | 200 µg/ml | $364.00 | |||
USE1 Antibody (D-1) Alexa Fluor® 647 | sc-398316 AF647 | 200 µg/ml | $364.00 | |||
USE1 Antibody (D-1) Alexa Fluor® 680 | sc-398316 AF680 | 200 µg/ml | $364.00 | |||
USE1 Antibody (D-1) Alexa Fluor® 790 | sc-398316 AF790 | 200 µg/ml | $364.00 |