Ubr1 Antibody (A-5): sc-515753
- Ubr1 Antibody (A-5) is a mouse monoclonal IgG1 κ Ubr1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 156-386 mapping within an internal region of Ubr1 of human origin
- Ubr1 Antibody (A-5) is recommended for detection of Ubr1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Ubr1 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ubr1 Antibody (A-5) for WB applications. These reagents are now offered in bundles with Ubr1 Antibody (A-5) (see ordering information below).
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Ubr1 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ubr1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Ubr1 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ubr1 plays a crucial role in the N-end rule pathway, which is integral to the ubiquitin-proteasome system responsible for protein degradation. This pathway links the half-life of proteins to the identity of their N-terminal residues, allowing for selective degradation of proteins based on their functional state or cellular context. Ubr1, also known as E3 alpha and N-recognin, recognizes specific N-terminal residues and catalyzes the attachment of ubiquitin to target proteins, marking them for degradation. Two zinc finger domains and a RING-H2 finger domain in Ubr1 are essential for substrate recognition capabilities, highlighting structural importance in mediating these interactions. Ubr1 is located on mouse chromosome 2 and human chromosome 15, with ubiquitous expression in adult tissues, particularly in skeletal muscle and heart, while in mouse embryos, Ubr1 is predominantly expressed in the branchial arches and limb buds, underscoring significance in developmental processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ubr1 Antibody (A-5) References:
- Bivalent inhibitor of the N-end rule pathway. | Kwon, YT., et al. 1999. J Biol Chem. 274: 18135-9. PMID: 10364269
- Altered activity, social behavior, and spatial memory in mice lacking the NTAN1p amidase and the asparagine branch of the N-end rule pathway. | Kwon, YT., et al. 2000. Mol Cell Biol. 20: 4135-48. PMID: 10805755
- Isolation, characterization, and partial purification of a novel ubiquitin-protein ligase, E3. Targeting of protein substrates via multiple and distinct recognition signals and conjugating enzymes. | Gonen, H., et al. 1996. J Biol Chem. 271: 302-10. PMID: 8550577
- The N-end rule pathway of protein degradation. | Varshavsky, A. 1997. Genes Cells. 2: 13-28. PMID: 9112437
- The mouse and human genes encoding the recognition component of the N-end rule pathway. | Kwon, YT., et al. 1998. Proc Natl Acad Sci U S A. 95: 7898-903. PMID: 9653112
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ubr1 Antibody (A-5) | sc-515753 | 200 µg/ml | $322.00 | |||
Ubr1 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-531498 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ubr1 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-525197 | 200 µg Ab, 40 µg BP | $361.00 | |||
Ubr1 Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-544593 | 200 µg Ab; 20 µg BP | $361.00 | |||
Ubr1 Antibody (A-5) AC | sc-515753 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ubr1 Antibody (A-5) HRP | sc-515753 HRP | 200 µg/ml | $322.00 | |||
Ubr1 Antibody (A-5) FITC | sc-515753 FITC | 200 µg/ml | $336.00 | |||
Ubr1 Antibody (A-5) PE | sc-515753 PE | 200 µg/ml | $349.00 | |||
Ubr1 Antibody (A-5) Alexa Fluor® 488 | sc-515753 AF488 | 200 µg/ml | $364.00 | |||
Ubr1 Antibody (A-5) Alexa Fluor® 546 | sc-515753 AF546 | 200 µg/ml | $364.00 | |||
Ubr1 Antibody (A-5) Alexa Fluor® 594 | sc-515753 AF594 | 200 µg/ml | $364.00 | |||
Ubr1 Antibody (A-5) Alexa Fluor® 647 | sc-515753 AF647 | 200 µg/ml | $364.00 | |||
Ubr1 Antibody (A-5) Alexa Fluor® 680 | sc-515753 AF680 | 200 µg/ml | $364.00 | |||
Ubr1 Antibody (A-5) Alexa Fluor® 790 | sc-515753 AF790 | 200 µg/ml | $364.00 |