TSHR Antibody (A10) recommended for detection of TSHR of human and porcine origin by WB, IP and IF

TSHR Antibody (A10): sc-57489

TSHR Antibody (A10) is rated 4.0 out of 5 by 1.
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Datasheets

    • TSHR Antibody (A10) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 22-35 of TSHR of human origin
    • recommended for detection of TSHR of human and porcine origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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For immunofluorescence staining on frozen tissue, is it recommended to previously fix the tissue with acetone or other product?

Asked by: Dani88
Thank you for your question. We recommend methanol or acetone for IF on frozen tissues. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2019-02-07

What is the recommended starting dilution for Western blot for sc-57489: TSHR (A10) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Positive result in western blot analysis Positive result in western blot analysis of TSHR expression in human thyroid tissue extract. -SCBT QC
Date published: 2013-09-27
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