TSHR Antibody (3B12): sc-53542

TSHR Antibody (3B12) is a mouse monoclonal IgG1 antibody that detects TSHR protein of mouse, rat, human, bovine, porcine, feline, and canine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). TSHR (3B12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The thyroid-stimulating hormone receptor (TSHR) plays a crucial role in thyroid function by mediating the effects of thyroid-stimulating hormone (TSH), a glycoprotein that regulates thyroid hormone synthesis and release. TSHR is primarily located on the surface of thyroid follicular cells, where TSHR initiates signaling cascades that lead to the production of thyroid hormones, which are essential for metabolism, growth, and development. Proper localization of TSHR is vital for function, as proper localization ensures that TSH can effectively bind to TSHR and activate downstream signaling pathways. Dysregulation of TSHR can lead to various thyroid disorders, including hyperthyroidism and hypothyroidism, highlighting the importance of TSHR in maintaining endocrine homeostasis. The third cytoplasmic loop of TSHR has been identified as critical for regulating inositol phosphate and cyclic adenosine monophosphate formation, further emphasizing the intricate mechanisms by which TSHR influences thyroid physiology.

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TSHR Antibody (3B12) References:

1. Subunit structure of thyrotropin receptors expressed on the cell surface.  |  Tanaka, K., et al. 1999. J Biol Chem. 274: 33979-84. PMID: 10567361
2. Effects of TSH receptor mutations on binding and biological activity of monoclonal antibodies and TSH.  |  Sanders, J., et al. 2006. Thyroid. 16: 1195-206. PMID: 17199429
3. A novel mutation in the thyrotropin (thyroid-stimulating hormone) receptor gene in a case of congenital hypothyroidism.  |  Jeziorowska, A., et al. 2006. Thyroid. 16: 1303-9. PMID: 17199441
4. Technology Insight: modern methods to monitor protein-protein interactions reveal functional TSH receptor oligomerization.  |  Persani, L., et al. 2007. Nat Clin Pract Endocrinol Metab. 3: 180-90. PMID: 17237844
5. Anti-thyroid-stimulating hormone receptor antibodies determined by second-generation assay.  |  Mankaï, A., et al. 2007. Clin Chem Lab Med. 45: 26-9. PMID: 17243910
6. The thyroid-stimulating hormone receptor: impact of thyroid-stimulating hormone and thyroid-stimulating hormone receptor antibodies on multimerization, cleavage, and signaling.  |  Latif, R., et al. 2009. Endocrinol Metab Clin North Am. 38: 319-41, viii. PMID: 19328414
7. Expression of BANCR promotes papillary thyroid cancer by targeting thyroid stimulating hormone receptor.  |  Zheng, H., et al. 2018. Oncol Lett. 16: 2009-2015. PMID: 30034553
8. The Absence of Thyroid-Stimulating Hormone Receptor Expression on Natural Killer T Cells: Implications for the Immune-Endocrine Interaction.  |  Adamska-Fita, E., et al. 2024. Int J Mol Sci. 25: PMID: 39518994
9. Substitutions of different regions of the third cytoplasmic loop of the thyrotropin (TSH) receptor have selective effects on constitutive, TSH-, and TSH receptor autoantibody-stimulated phosphoinositide and 3',5'-cyclic adenosine monophosphate signal generation.  |  Kosugi, S., et al. 1993. Mol Endocrinol. 7: 1009-20. PMID: 7901757
10. The rat TSHbeta gene contains distinct response elements for regulation by retinoids and thyroid hormone.  |  Breen, JJ., et al. 1997. Mol Cell Endocrinol. 131: 137-46. PMID: 9296372
11. Understanding the thyrotropin receptor function-structure relationship.  |  Sanders, J., et al. 1997. Baillieres Clin Endocrinol Metab. 11: 451-79. PMID: 9532334
12. Functional homodimeric glycoprotein hormones: implications for hormone action and evolution.  |  Moyle, WR., et al. 1998. Chem Biol. 5: 241-54. PMID: 9646940

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TSHR Antibody (3B12) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-TSHR Antibody (3B12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TSHR Antibody (3B12) Specifications

Alternate Product Name: Thyroid Stimulating Hormone Receptor

Product Clone Name: 3B12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 1-415

Epitope Target: TSHR

Epitope Species: human

Product Reactivity/Detection: TSHR

Species Reactivity/Detection: mouse, rat, human, bovine, porcine, feline, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for TSHR Antibody (3B12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin