TS Antibody (F-7): sc-376161
- TS Antibody (F-7) is a mouse monoclonal IgG2b κ, cited in 8 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 99-127 within an internal region of TS of human origin
- recommended for detection of TS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and porcine
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TS Antibody (F-7) for WB and IHC(P) applications. These reagents are now offered in bundles with TS Antibody (F-7) (see ordering information below).
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TS Antibody (F-7) is a mouse monoclonal IgG2b antibody that detects thymidylate synthase (TS) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TS plays a crucial role in DNA biosynthesis by catalyzing the conversion of 2′-deoxyuridine-5′-monophosphate (dUMP) to 2′-deoxythymidine-5′-monophosphate (dTMP) using 5,10-methylenetetrahydrofolate as a cofactor, making TS essential for cell proliferation and survival. TS′s importance extends beyond enzymatic function as an RNA-binding protein that can interact with its own mRNA, forming a ribonucleoprotein complex that associates with other cellular mRNAs, including those of the p53 tumor suppressor gene and Myc transcription factors. This interaction highlights TS′s role in regulating gene expression and cellular responses to stress, particularly in cancer cells that are highly sensitive to thymidine depletion. Inhibition of TS activity can lead to impaired DNA replication and increased cell death, making TS a significant target for chemotherapy in rapidly dividing cancer cells. Anti-TS antibody (F-7) serves as a valuable tool for researchers studying cancer biology mechanisms and the therapeutic potential of targeting TS in cancer treatment.
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TS Antibody (F-7) References:
- Thymidylate synthase as a translational regulator of cellular gene expression. | Liu, J., et al. 2002. Biochim Biophys Acta. 1587: 174-82. PMID: 12084459
- Thymidylate synthase pharmacogenetics in colorectal cancer. | Marsh, S. and McLeod, HL. 2001. Clin Colorectal Cancer. 1: 175-8; discussion 179-81. PMID: 12450432
- Translational autoregulation of thymidylate synthase and dihydrofolate reductase. | Tai, N., et al. 2004. Front Biosci. 9: 2521-6. PMID: 15353304
- Inhibition of thymidylate synthase affects neural tube development in mice. | Wang, X., et al. 2018. Reprod Toxicol. 76: 17-25. PMID: 29258758
- Formaldehyde regulates tetrahydrofolate stability and thymidylate synthase catalysis. | Chen, X., et al. 2021. Chem Commun (Camb). 57: 5778-5781. PMID: 33997872
- Thymidylate synthase is essential for efficient HIV-1 replication in macrophages. | Desrosiers, V., et al. 2021. Virology. 561: 47-57. PMID: 34146963
- Germline thymidylate synthase deficiency impacts nucleotide metabolism and causes dyskeratosis congenita. | Tummala, H., et al. 2022. Am J Hum Genet. 109: 1472-1483. PMID: 35931051
- Dynamic allostery in substrate binding by human thymidylate synthase. | Bonin, JP., et al. 2022. Elife. 11: PMID: 36200982
- Destabilizers of the thymidylate synthase homodimer accelerate its proteasomal degradation and inhibit cancer growth. | Costantino, L., et al. 2022. Elife. 11: PMID: 36475542
- Thymidylate synthase disruption to limit cell proliferation in cell therapies. | Sartori-Maldonado, R., et al. 2024. Mol Ther. 32: 2535-2548. PMID: 38867450
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TS Antibody (F-7) | sc-376161 | 200 µg/ml | $322.00 | |||
TS Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-537975 | 200 µg Ab; 10 µg BP | $361.00 | |||
TS Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-535471 | 200 µg Ab; 40 µg BP | $361.00 | |||
TS Antibody (F-7): m-IgG2b BP-HRP Bundle | sc-549907 | 200 µg Ab; 10 µg BP | $361.00 | |||
TS (F-7) Neutralizing Peptide | sc-376161 P | 100 µg/0.5 ml | $69.00 |