TREX-1 Antibody (E-6): sc-271870

TREX-1 Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects TREX-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TREX-1 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TREX-1, also known as three prime repair exonuclease 1, trophoblast expressed 1, CRV, AGS1, AGS5, DRN3, HERNS, or DNase III, is a crucial member of the exonuclease superfamily and plays a significant role in DNA metabolism and repair. TREX-1 is predominantly expressed in the spleen, liver, thymus, colon, heart, brain, and small intestine, where TREX-1 localizes to the nucleus and functions as a homodimer. TREX-1 is an exonuclease that requires magnesium as a cofactor for activity, demonstrating a preference for double-stranded DNA and operating in the 3′-5′ direction to yield nucleoside 5′-phosphates. Notably, TREX-1 interacts with the SET complex, an endoplasmic reticulum-associated complex, and is involved in granzyme A-mediated apoptosis, highlighting its importance in cellular processes. Mutations or defects in the gene encoding TREX-1 have been linked to several diseases, including systemic lupus erythematosus, chilblain lupus, Aicardi-Goutieres syndrome types 1 and 5, and autosomal dominant retinal vasculopathy with cerebral leukodystrophy, underscoring TREX-1′s critical role in maintaining genomic integrity and cellular health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TREX-1 Antibody (E-6) References:

1. Recruitment of the human TREX complex to mRNA during splicing.  |  Masuda, S., et al. 2005. Genes Dev. 19: 1512-7. PMID: 15998806
2. ATRIP associates with replication protein A-coated ssDNA through multiple interactions.  |  Namiki, Y. and Zou, L. 2006. Proc Natl Acad Sci U S A. 103: 580-5. PMID: 16407120
3. Mismatch repair proteins as sensors of alkylation DNA damage.  |  Wang, JY. and Edelmann, W. 2006. Cancer Cell. 9: 417-8. PMID: 16766259
4. The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death.  |  Chowdhury, D., et al. 2006. Mol Cell. 23: 133-42. PMID: 16818237
5. Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 cause Aicardi-Goutières syndrome at the AGS1 locus.  |  Crow, YJ., et al. 2006. Nat Genet. 38: 917-20. PMID: 16845398
6. Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 are associated with systemic lupus erythematosus.  |  Lee-Kirsch, MA., et al. 2007. Nat Genet. 39: 1065-7. PMID: 17660818
7. DNA mismanagement leads to immune system oversight.  |  Coscoy, L. and Raulet, DH. 2007. Cell. 131: 836-8. PMID: 18045527
8. Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease.  |  Yang, YG., et al. 2007. Cell. 131: 873-86. PMID: 18045533
9. TREX1 polymorphisms associated with autoantibodies in patients with systemic lupus erythematosus.  |  Hur, JW., et al. 2008. Rheumatol Int. 28: 783-9. PMID: 18092167
10. Trex1 regulates lysosomal biogenesis and interferon-independent activation of antiviral genes.  |  Hasan, M., et al. 2013. Nat Immunol. 14: 61-71. PMID: 23160154
11. TREX1 is expressed by microglia in normal human brain and increases in regions affected by ischemia.  |  Kothari, PH., et al. 2018. Brain Pathol. 28: 806-821. PMID: 30062819
12. TREX1 cytosolic DNA degradation correlates with autoimmune disease and cancer immunity.  |  Fang, L., et al. 2023. Clin Exp Immunol. 211: 193-207. PMID: 36745566

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TREX-1 Antibody (E-6) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-TREX-1 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TREX-1 Antibody (E-6) Specifications

Alternate Product Name: TREX-1

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 290-369

Epitope Target: TREX-1

Epitope Species: human

Product Reactivity/Detection: three prime repair exonuclease 1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for TREX-1 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred TREX-1 antibody is TREX-1 Antibody (C-11): sc-133112.