TREX-1 Antibody (C-11): sc-133112

TREX-1 Antibody (C-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects three prime repair exonuclease 1 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TREX-1 (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Three prime repair exonuclease 1, also known as trophoblast expressed 1, CRV, AGS1, AGS5, DRN3, HERNS, or DNase III, is a crucial member of the exonuclease superfamily and plays a significant role in DNA metabolism and repair. Three prime repair exonuclease 1 is predominantly expressed in the spleen, liver, thymus, colon, heart, brain, and small intestine, where TREX-1 localizes to the nucleus and functions as a homodimer. TREX-1 is an exonuclease that requires magnesium as a cofactor for activity, demonstrating a preference for double-stranded DNA and operating in the 3′-5′ direction to yield nucleoside 5′-phosphates. TREX-1 interacts with the SET complex, an endoplasmic reticulum-associated complex, and is involved in granzyme A-mediated apoptosis, highlighting TREX-1′s importance in cellular processes. Mutations or defects in the gene encoding TREX-1 have been linked to several diseases, including systemic lupus erythematosus, chilblain lupus, Aicardi-Goutieres syndrome types 1 and 5, and autosomal dominant retinal vasculopathy with cerebral leukodystrophy, underscoring TREX-1′s critical role in maintaining genomic integrity and cellular health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TREX-1 Antibody (C-11) References:

1. Human disease phenotypes associated with mutations in TREX1.  |  Rice, GI., et al. 2015. J Clin Immunol. 35: 235-43. PMID: 25731743
2. DNA exonuclease Trex1 regulates radiotherapy-induced tumour immunogenicity.  |  Vanpouille-Box, C., et al. 2017. Nat Commun. 8: 15618. PMID: 28598415
3. TREX1 As a Potential Therapeutic Target for Autoimmune and Inflammatory Diseases.  |  Tao, SS., et al. 2019. Curr Pharm Des. 25: 3239-3247. PMID: 31475890
4. Compromised nuclear envelope integrity drives TREX1-dependent DNA damage and tumor cell invasion.  |  Nader, GPF., et al. 2021. Cell. 184: 5230-5246.e22. PMID: 34551315
5. TREX1 Deficiency Induces ER Stress-Mediated Neuronal Cell Death by Disrupting Ca2+ Homeostasis.  |  Halder, D., et al. 2022. Mol Neurobiol. 59: 1398-1418. PMID: 34997539
6. TREX1 Inactivation Unleashes Cancer Cell STING-Interferon Signaling and Promotes Antitumor Immunity.  |  Tani, T., et al. 2024. Cancer Discov. 14: 752-765. PMID: 38227896
7. Adaptive inhibition of CGAS signaling by TREX1.  |  Lira, MC., et al. 2024. Trends Cancer. 10: 177-179. PMID: 38355355
8. Intratumoral TREX1 Induction Promotes Immune Evasion by Limiting Type I IFN.  |  Toufektchan, E., et al. 2024. Cancer Immunol Res. 12: 673-686. PMID: 38408184
9. The Exonuclease TREX1 Constitutes an Innate Immune Checkpoint Limiting cGAS/STING-Mediated Antitumor Immunity.  |  Lim, J., et al. 2024. Cancer Immunol Res. 12: 663-672. PMID: 38489753
10. Targeting TREX1 Induces Innate Immune Response in Drug-Resistant Small-Cell Lung Cancer.  |  Murayama, T., et al. 2024. Cancer Res Commun. 4: 2399-2414. PMID: 39177280

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TREX-1 Antibody (C-11) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-TREX-1 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TREX-1 Antibody (C-11) Specifications

Alternate Product Name: TREX-1

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 201-314

Epitope Target: TREX-1

Epitope Species: mouse

Product Reactivity/Detection: three prime repair exonuclease 1

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for TREX-1 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred TREX-1 antibody is TREX-1 Antibody (E-6): sc-271870.