Transketolase Antibody (H-7): sc-390179
- Transketolase Antibody (H-7) is a mouse monoclonal IgG2a κ Transketolase antibody, cited in 14 publications, provided at 200 µg/ml
- raised against amino acids 261-310 mapping within an internal region of Transketolase of human origin
- Transketolase Antibody (H-7) is recommended for detection of Transketolase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Transketolase Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Transketolase Antibody (H-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Transketolase Antibody (H-7) (see ordering information below).
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Transketolase Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Transketolase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Transketolase antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Transketolase (TKT) is a vital enzyme in the pentose phosphate pathway, where TKT catalyzes the transfer of two-carbon units between sugars, facilitating the conversion of glucose into ribose-5-phosphate, which is essential for nucleotide synthesis. This function is particularly important in rapidly dividing cells, such as those found in tumors, as TKT provides the necessary building blocks for DNA and RNA synthesis, thereby supporting cell proliferation. Additionally, TKT plays a protective role against oxidative stress by generating NADPH, which is crucial for maintaining cellular redox balance. TKT is predominantly located in the cytoplasm of various tissues, including the liver, kidney, and small intestine, highlighting TKT′s significance in metabolic regulation and cellular health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Transketolase Antibody (H-7) References:
- Functional flexibility of the transketolase molecule. | Kochetov, GA. 2001. Biochemistry (Mosc). 66: 1077-85. PMID: 11736629
- Benfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathy. | Hammes, HP., et al. 2003. Nat Med. 9: 294-9. PMID: 12592403
- Uncoupling keratocyte loss of corneal crystallin from markers of fibrotic repair. | Stramer, BM. and Fini, ME. 2004. Invest Ophthalmol Vis Sci. 45: 4010-5. PMID: 15505050
- Donor substrate regulation of transketolase. | Esakova, OA., et al. 2004. Eur J Biochem. 271: 4189-94. PMID: 15511224
- High-dose thiamine therapy counters dyslipidaemia in streptozotocin-induced diabetic rats. | Babaei-Jadidi, R., et al. 2004. Diabetologia. 47: 2235-46. PMID: 15662560
- Outbreak of life-threatening thiamine deficiency in infants in Israel caused by a defective soy-based formula. | Fattal-Valevski, A., et al. 2005. Pediatrics. 115: e233-8. PMID: 15687431
- In situ localization of transketolase activity in epithelial cells of different rat tissues and subcellularly in liver parenchymal cells. | Boren, J., et al. 2006. J Histochem Cytochem. 54: 191-9. PMID: 16116031
- Effects of transketolase cofactors on its conformation and stability. | Esakova, OA., et al. 2005. Life Sci. 78: 8-13. PMID: 16125202
- The mouse transketolase (TKT) gene: cloning, characterization, and functional promoter analysis. | Salamon, C., et al. 1998. Genomics. 48: 209-20. PMID: 9521875
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Transketolase Antibody (H-7) | sc-390179 | 200 µg/ml | $316.00 | |||
Transketolase Antibody (H-7): m-IgG Fc BP-HRP Bundle | sc-530086 | 200 µg Ab; 10 µg BP | $354.00 | |||
Transketolase Antibody (H-7): m-IgGκ BP-HRP Bundle | sc-523437 | 200 µg Ab, 40 µg BP | $354.00 | |||
Transketolase Antibody (H-7): m-IgG2a BP-HRP Bundle | sc-547429 | 200 µg Ab; 10 µg BP | $354.00 | |||
Transketolase Antibody (H-7) AC | sc-390179 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Transketolase Antibody (H-7) HRP | sc-390179 HRP | 200 µg/ml | $316.00 | |||
Transketolase Antibody (H-7) FITC | sc-390179 FITC | 200 µg/ml | $330.00 | |||
Transketolase Antibody (H-7) PE | sc-390179 PE | 200 µg/ml | $343.00 | |||
Transketolase Antibody (H-7) Alexa Fluor® 488 | sc-390179 AF488 | 200 µg/ml | $357.00 | |||
Transketolase Antibody (H-7) Alexa Fluor® 546 | sc-390179 AF546 | 200 µg/ml | $357.00 | |||
Transketolase Antibody (H-7) Alexa Fluor® 594 | sc-390179 AF594 | 200 µg/ml | $357.00 | |||
Transketolase Antibody (H-7) Alexa Fluor® 647 | sc-390179 AF647 | 200 µg/ml | $357.00 | |||
Transketolase Antibody (H-7) Alexa Fluor® 680 | sc-390179 AF680 | 200 µg/ml | $357.00 | |||
Transketolase Antibody (H-7) Alexa Fluor® 790 | sc-390179 AF790 | 200 µg/ml | $357.00 |