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TRAF2 Antibody (F-2): sc-136999

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Datasheets
  • TRAF2 Antibody (F-2) is a mouse monoclonal IgG1 κ TRAF2 antibody, cited in 20 publications, provided at 200 µg/ml
  • raised against amino acids 1-249 of TRAF2 of human origin
  • TRAF2 Antibody (F-2) is recommended for detection of TRAF2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-TRAF2 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRAF2 Antibody (F-2) for WB and IHC(P) applications. These reagents are now offered in bundles with TRAF2 Antibody (F-2) (see ordering information below).

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TRAF2 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRAF2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-TRAF2 antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRAF2 plays a crucial role in mediating tumor necrosis factor (TNF) signaling, primarily through interaction with TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF receptor 2 (TNF-R2). These receptors are integral members of the TNF receptor superfamily, which includes important proteins like FAS and CD40. Identification of TRAF2, along with homolog TRAF1, has provided significant insights into TNF receptor-mediated signaling mechanisms, as both proteins form heterodimeric complexes that associate with the cytoplasmic domain of TNF-R2. TRAF2 is essential for recruiting downstream signaling molecules, which ultimately leads to activation of various cellular responses, including inflammation and apoptosis. Understanding TRAF2 function is vital, as dysregulation of TNF signaling is implicated in numerous diseases, including autoimmune disorders and cancer, making TRAF2 a potential therapeutic target.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

TRAF2 Antibody (F-2)

sc-136999
200 µg/ml
$322.00

TRAF2 Antibody (F-2): m-IgG Fc BP-HRP Bundle

sc-528835
200 µg Ab; 10 µg BP
$361.00

TRAF2 Antibody (F-2): m-IgGκ BP-HRP Bundle

sc-521388
200 µg Ab, 40 µg BP
$361.00

TRAF2 Antibody (F-2): m-IgG1 BP-HRP Bundle

sc-543162
200 µg Ab; 20 µg BP
$361.00

TRAF2 Antibody (F-2) AC

sc-136999 AC
500 µg/ml, 25% agarose
$424.00

TRAF2 Antibody (F-2) HRP

sc-136999 HRP
200 µg/ml
$322.00

TRAF2 Antibody (F-2) FITC

sc-136999 FITC
200 µg/ml
$336.00

TRAF2 Antibody (F-2) PE

sc-136999 PE
200 µg/ml
$349.00

TRAF2 Antibody (F-2) Alexa Fluor® 488

sc-136999 AF488
200 µg/ml
$364.00

TRAF2 Antibody (F-2) Alexa Fluor® 546

sc-136999 AF546
200 µg/ml
$364.00

TRAF2 Antibody (F-2) Alexa Fluor® 594

sc-136999 AF594
200 µg/ml
$364.00

TRAF2 Antibody (F-2) Alexa Fluor® 647

sc-136999 AF647
200 µg/ml
$364.00

TRAF2 Antibody (F-2) Alexa Fluor® 680

sc-136999 AF680
200 µg/ml
$364.00

TRAF2 Antibody (F-2) Alexa Fluor® 790

sc-136999 AF790
200 µg/ml
$364.00

购买了此抗体做IF。在HCT116 WT和HCT116 TRAF2 KO 细胞中进行染色,两者都染出来了,且亮度没有明显差异。细胞已进行WB TRAF2 含量检测,没有问题。染色时,所有试剂都是新配的,重复了两次结果一致。请问有什么解决方法吗。

Asked by: xuan ke
感谢您的提问。请联系我们的技术支持部门了解更多信息。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-01-07

Can mouse monoclonal antibody be used for WB of mouse B cell sample? Wouldn't the anti-mouse-HRP secondary antibody binds to the native IgG in B cells too? Does the anti-mouse secondary antibody only recognizes non-denatured form of mouse IgG?

Asked by: MJann
Thank you for your question. Generally, there are no issues with secondary anti-mouse antibodies being used in western blots with samples from mouse. However, if you are concerned about unspecific binding of secondary antibodies, TRAF2 Antibody (F-2): sc-136999 and many other antibodies in our portfolio are available as direct conjugates, which do not require a secondary antibody.
Answered by: Tech Support Europe
Date published: 2021-03-05

Can TRAF2 (F-2): sc-136999 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TRAF2 (F-2): sc-136999 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good antibodyHighly specific detection of endogenous and over expressed TRAF2 in WB; tested in HeLa cells (whole lysats and IP samples)
Date published: 2020-09-09
Rated 5 out of 5 by from Very good in WBTRAF2 bands were detectable in lysates of A549 cells and 293T cells by WB at dilution of 1/1000.
Date published: 2019-09-26
Rated 5 out of 5 by from Great feutureHigh specificity and nice product from Santa Cruz.
Date published: 2017-08-21
Rated 4 out of 5 by from 功能较好最近在Santa上申请的这一只抗体,跑wb有杂带出现了,抗体量少,加大上样量感觉条带也不是很黑的,而且背景也是特别黑,尝试用牛奶或者bsa封闭都没有改善,所以具体不知道是怎么回事啊
Date published: 2017-08-20
Rated 5 out of 5 by from worked well in WBWe used this antibody in a western blot and got very nice results in human cells and mef cells.
Date published: 2017-08-09
Rated 5 out of 5 by from Very good in WB in HelaIt is a very good Ab for WB in hela whole cell lysis 1:1000
Date published: 2017-07-30
Rated 5 out of 5 by from specific clear band in cell lysate by westernWe have tested the antibody sample for western blotting for expression in cell lysate from human cell lines. The western blot showed only one specific, clear band.
Date published: 2017-07-20
Rated 5 out of 5 by from Strong band in WBIt works very well in WB and it has strong band at 1:1000 dilution
Date published: 2017-07-12
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TRAF2 Antibody (F-2) is rated 4.9 out of 5 by 15.
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