Tom22 Antibody (1C9-2): sc-58308

Tom22 Antibody (1C9-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tom22 protein of mouse, rat, human, and monkey origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Tom22 (1C9-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tom22 protein plays a crucial role in mitochondrial function as a component of the mitochondrial preprotein translocase of the outer membrane (Tom) complex, which is essential for importing nucleus-encoded precursor proteins into mitochondria. This protein complex is vital for cellular energy production and metabolism, ensuring proteins necessary for mitochondrial function are correctly imported and integrated into the organelle. Tom22 serves as a receptor for preproteins and acts as a docking point for Tom20 and the general import pore (GIP), facilitating protein translocation across the mitochondrial outer membrane. Tom22′s structural characteristics, including N-terminal negatively charged region and putative transmembrane domain, are important for interaction with other components of the import machinery, ensuring efficient protein transport. The gene encoding human Tom22 is located on chromosome 22q12-q13, highlighting evolutionary conservation and significance across species.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Tom22 Antibody (1C9-2) References:

1. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase.  |  van Wilpe, S., et al. 1999. Nature. 401: 485-9. PMID: 10519552
2. TOM22, a core component of the mitochondria outer membrane protein translocation pore, is a mitochondrial receptor for the proapoptotic protein Bax.  |  Bellot, G., et al. 2007. Cell Death Differ. 14: 785-94. PMID: 17096026
3. Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import.  |  Yamano, K., et al. 2008. J Biol Chem. 283: 3799-807. PMID: 18063580
4. Glucose-induced regulation of protein import receptor Tom22 by cytosolic and mitochondria-bound kinases.  |  Gerbeth, C., et al. 2013. Cell Metab. 18: 578-87. PMID: 24093680
5. An Outer Mitochondrial Translocase, Tom22, Is Crucial for Inner Mitochondrial Steroidogenic Regulation in Adrenal and Gonadal Tissues.  |  Rajapaksha, M., et al. 2016. Mol Cell Biol. 36: 1032-47. PMID: 26787839
6. Mitochondrial inner-membrane protease Yme1 degrades outer-membrane proteins Tom22 and Om45.  |  Wu, X., et al. 2018. J Cell Biol. 217: 139-149. PMID: 29138251
7. Porin Associates with Tom22 to Regulate the Mitochondrial Protein Gate Assembly.  |  Sakaue, H., et al. 2019. Mol Cell. 73: 1044-1055.e8. PMID: 30738703
8. Structural basis of Tom20 and Tom22 cytosolic domains as the human TOM complex receptors.  |  Su, J., et al. 2022. Proc Natl Acad Sci U S A. 119: e2200158119. PMID: 35733257
9. The membrane insertion of the pro-apoptotic protein Bax is a Tom22-dependent multi-step process: a study in nanodiscs.  |  Rouchidane Eyitayo, A., et al. 2024. Cell Death Discov. 10: 335. PMID: 39043635
10. Role of the negative charges in the cytosolic domain of TOM22 in the import of precursor proteins into mitochondria.  |  Nargang, FE., et al. 1998. Mol Cell Biol. 18: 3173-81. PMID: 9584158

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom22 Antibody (1C9-2) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Tom22 Antibody (1C9-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tom22 Antibody (1C9-2) Specifications

Alternate Product Name: Tom22

Product Clone Name: 1C9-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Tom22

Epitope Species: monkey

Product Reactivity/Detection: Tom22

Species Reactivity/Detection: mouse, rat, human, monkey

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Tom22 Antibody (1C9-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Tom22 antibody is Tom22 Antibody (J-31): sc-101286.