TNAP Antibody (3H415): sc-73524

TNAP Antibody (3H415) is a mouse monoclonal IgG1 antibody that detects tissue non-specific alkaline phosphatase (TNAP) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). TNAP is a crucial enzyme that plays a significant role in bone mineralization, functioning alongside plasma cell membrane glycoprotein-1 to facilitate the deposition of minerals in the bone matrix. This process is vital for maintaining bone integrity and strength, and any disruption can lead to serious skeletal disorders. Notably, mutations or deficiencies in the TNAP gene are directly linked to hypophosphatasia, a rare genetic condition characterized by impaired bone mineralization and various skeletal abnormalities. TNAP is widely expressed in tissues such as the liver, kidney, and bone, highlighting TNAP′s importance in multiple physiological processes. The human gene encoding TNAP is located on chromosome 1p36.12, and understanding TNAP′s function and interactions is essential for elucidating the mechanisms underlying bone health and disease. Anti-TNAP antibody (3H415) provides a reliable tool for researchers investigating the role of this enzyme in both normal physiology and pathological conditions.

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TNAP Antibody (3H415) References:

1. Investigation of the role of ENPP1 and TNAP genes in chondrocalcinosis.  |  Zhang, Y., et al. 2007. Rheumatology (Oxford). 46: 586-9. PMID: 17043047
2. Inhibitors of tissue-nonspecific alkaline phosphatase (TNAP): from hits to leads.  |  Teriete, P., et al. 2013. Methods Mol Biol. 1053: 85-101. PMID: 23860648
3. The Role of Tissue Non-specific Alkaline Phosphatase (TNAP) in Neurodegenerative Diseases: Alzheimer's Disease in the Focus.  |  Kellett, KA. and Hooper, NM. 2015. Subcell Biochem. 76: 363-74. PMID: 26219720
4. Increased activity of TNAP compensates for reduced adenosine production and promotes ectopic calcification in the genetic disease ACDC.  |  Jin, H., et al. 2016. Sci Signal. 9: ra121. PMID: 27965423
5. Overexpression of tissue-nonspecific alkaline phosphatase (TNAP) in endothelial cells accelerates coronary artery disease in a mouse model of familial hypercholesterolemia.  |  Romanelli, F., et al. 2017. PLoS One. 12: e0186426. PMID: 29023576
6. HLA-B27-mediated activation of TNAP phosphatase promotes pathogenic syndesmophyte formation in ankylosing spondylitis.  |  Liu, CH., et al. 2019. J Clin Invest. 129: 5357-5373. PMID: 31682238
7. Loss of tissue-nonspecific alkaline phosphatase (TNAP) enzyme activity in cerebral microvessels is coupled to persistent neuroinflammation and behavioral deficits in late sepsis.  |  Nwafor, DC., et al. 2020. Brain Behav Immun. 84: 115-131. PMID: 31778743
8. Synthesis, characterization, in vitro tissue-nonspecific alkaline phosphatase (TNAP) and intestinal alkaline phosphatase (IAP) inhibition studies and computational evaluation of novel thiazole derivatives.  |  Aziz, H., et al. 2020. Bioorg Chem. 102: 104088. PMID: 32711087
9. Gene Therapy Using Adeno-Associated Virus Serotype 8 Encoding TNAP-D10 Improves the Skeletal and Dentoalveolar Phenotypes in Alpl-/- Mice.  |  Kinoshita, Y., et al. 2021. J Bone Miner Res. 36: 1835-1849. PMID: 34076297
10. Azomethine-clubbed thiazoles as human tissue non-specific alkaline phosphatase (h-TNAP) and intestinal alkaline phosphatase (h-IAP) Inhibitors: kinetics and molecular docking studies.  |  Saeed, A., et al. 2022. Mol Divers. 26: 3241-3254. PMID: 35083622

The preferred TNAP antibody is TNAP Antibody (TRA-2-39): sc-21708.