TNAP Antibody (F-4): sc-166261

TNAP Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects tissue non-specific alkaline phosphatase (TNAP) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-TNAP antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TNAP (F-4) monoclonal antibody recognizes a protein that plays a crucial role in bone mineralization, functioning alongside plasma cell membrane glycoprotein-1 to facilitate mineral deposition in the bone matrix. This process is vital for maintaining bone integrity and strength, and any disruption can lead to significant skeletal issues. Mutations or deficiencies in TNAP are directly associated with hypophosphatasia, a rare genetic disorder characterized by impaired bone mineralization and skeletal deformities. TNAP (F-4) antibody targets a protein encoded by a gene located on chromosome 1p36.1-p34, with widespread expression in tissues such as liver, kidney, bone, stomach, and colon, underscoring TNAP′s importance in various physiological processes.

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TNAP Antibody (F-4) References:

1. Alkaline phosphatase activity in normal and inflamed dental pulps.  |  Spoto, G., et al. 2001. J Endod. 27: 180-2. PMID: 11487147
2. On the interaction of the vanadyl(IV) cation with lactose: inhibition effects of vanadyl(IV)/monosaccharide and disaccharide complexes upon alkaline phosphatase activity.  |  Etcheverry, SB., et al. 2001. Biol Trace Elem Res. 84: 227-38. PMID: 11817692
3. Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage.  |  Miao, D. and Scutt, A. 2002. J Histochem Cytochem. 50: 333-40. PMID: 11850436
4. Effects of various fibrates on serum alkaline phosphatase activity.  |  Ganotakis, E., et al. 2002. Atherosclerosis. 165: 187-8. PMID: 12208487
5. Potentiating role of IGFBP-2 on IGF-II-stimulated alkaline phosphatase activity in differentiating osteoblasts.  |  Palermo, C., et al. 2004. Am J Physiol Endocrinol Metab. 286: E648-57. PMID: 14665441
6. Role of albumin in osteoblastic cells: enhancement of cell proliferation and suppression of alkaline phosphatase activity.  |  Ishida, K. and Yamaguchi, M. 2004. Int J Mol Med. 14: 1077-81. PMID: 15547677
7. Calcification of human valve interstitial cells is dependent on alkaline phosphatase activity.  |  Mathieu, P., et al. 2005. J Heart Valve Dis. 14: 353-7. PMID: 15974530
8. Alkaline phosphatase activity in gingival crevicular fluid during canine retraction.  |  Batra, P., et al. 2006. Orthod Craniofac Res. 9: 44-51. PMID: 16420274
9. Tissue Non-specific Alkaline Phosphatase (TNAP) in Vessels of the Brain.  |  Deracinois, B., et al. 2015. Subcell Biochem. 76: 125-51. PMID: 26219710
10. Multiple Functions of MSCA-1/TNAP in Adult Mesenchymal Progenitor/Stromal Cells.  |  Estève, D., et al. 2016. Stem Cells Int. 2016: 1815982. PMID: 26839555
11. TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways.  |  Gao, L., et al. 2020. Cell Death Dis. 11: 44. PMID: 31969558
12. Leucocyte alkaline phosphatase activity in patients with squamous cell carcinoma.  |  Bisht, D., et al. 1997. Indian J Pathol Microbiol. 40: 147-51. PMID: 9257500

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TNAP Antibody (F-4) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-TNAP Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TNAP Antibody (F-4) Specifications

Alternate Product Name: TNAP

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 18-317

Epitope Target: TNAP

Epitope Species: human

Product Reactivity/Detection: TNAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for TNAP Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin