Anti-TNAP Antibody (F-4) recommended for detection of TNAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TNAP Antibody (F-4): sc-166261

Anti-TNAP Antibody (F-4) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-TNAP Antibody (F-4) is a mouse monoclonal IgG1 κ TNAP antibody, cited in 9 publications, provided at 200 µg/ml
    • raised against amino acids 18-317 mapping near the N-terminus of TNAP of human origin
    • Anti-TNAP Antibody (F-4) is recommended for detection of TNAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TNAP Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TNAP (F-4): sc-166261.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TNAP Antibody (F-4) for WB and IHC(P) applications. These reagents are now offered in bundles with TNAP Antibody (F-4) (see ordering information below).
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I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 4 out of 5 by from Good for WB Works well in 1:200 dilution in western blot, using HeLa whole cell lysate as a sample.
Date published: 2018-02-23
Rated 5 out of 5 by from Top notch! The (F-4) clone is a top notch monoclonal antibody validated in multiple research applications including WB and IHC(P).
Date published: 2017-01-07
Rated 5 out of 5 by from Produced positive Western Blot data of TNAP Produced positive Western Blot data of TNAP expression in non-transfected 293T, human TNAP transfected 293T and NTERA-2 cl. -SCBT QC
Date published: 2013-03-31
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